大孔吸附树脂法纯化甲基莲心碱的工艺研究  

Separation and purification of neferine in Nelumbinis Plumula by resins

作　　者：吕晶[1] 金磊 王润东 王祯 李枝旺 薛舒婷 刘小宇 赵乃璇 陈长兰[1] LYU Jing;JIN Lei;WANG Run-dong;WANG Zhen;LI Zhi-wang;XUE Shu-ting;LIU Xiao-yu;ZHAO Nai-xuan;CHEN Chang-lan(School of Pharmaceutical Science,Liaoning University,Shenyang 110036,China)

机构地区：[1]辽宁大学药学院

出　　处：《哈尔滨商业大学学报（自然科学版）》2019年第5期522-525,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：辽宁省教育厅科学研究一般项目(LYB201610);辽宁大学青年科研基金项目(LDQN2015003)

摘　　要：筛选适用于分离纯化甲基莲心碱的大孔吸附树脂.考察吸附性能较好的HPD-600、D101、AB-8三种大孔吸附树脂对甲基莲心碱的解吸附能力及洗脱参数,并用HPLC-UV定量分析甲基莲心碱的含量.D101树脂对甲基莲心碱有较好的吸附分离效果.莲子心提取液调节pH值至11,经大孔吸附树脂吸附后,分别用4BV的蒸馏水、5BV的60%乙醇洗脱,洗脱率为83.11%,收率为34.6%,甲基莲心碱纯度从16.8%提高到70.0%,纯度提高了约3.3倍.该工艺简单可行,分离效果好,适合甲基莲心碱的分离纯化.To screen the macroporous resin for the separation and purification of neferine from Nelumbinis Plumula. The resins such as HPD-600, D101, AB-8 were studied for their adsorption capability and elution parameters. The contents of neferine were determined by HPLC-UV. Results D101 resin had a preferable adsorptive and separative effect for neferine in Nelumbinis Plumula. The preferable desorption ratio could be obtained by using water (4BV, pH 9) and 60% ethanol (5BV) as eluting solvent. Under these conditions, the yield was 0.65 % and the content of neferine in Nelumbinis Plumula was 70.0% greater than initial content that was 16.8%. The method is easy and possesses the popularization potential and good efficacy. It could be used for the separation and purification of neferine in Nelumbinis Plumula.

关 键 词：莲子心 生物碱 甲基莲心碱 大孔吸附树脂 纯化 工艺优化 

分 类 号：R284.2[医药卫生—中药学]