单细胞蛋白定量检测方法综述  

Review of quantitative detection methods of single-cell proteins

作　　者：李秀锋[1,2] 范蓓媛 刘力行[1,2] 王军波 陈德勇 陈健[1] LI Xiufeng;FAN Beiyuan;LIU Lixing;WANG Junbo;CHEN Deyong;CHEN Jian(State Key Laboratory of Transducer Technology, Institute of Electronics, Chiness Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences ,Beijing 100039,China)

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100190 [2]中国科学院大学,北京100039

出　　处：《传感器与微系统》2019年第10期1-3,7,共4页Transducer and Microsystem Technologies

基　　金：国家重点基础研究发展规划(“973”计划)资助项目(2014CB744600);国家自然科学基金资助项目(61431019,61671430)

摘　　要：单细胞水平的蛋白检测方法能够精细地反映生命事件的发生过程,同时对于少量样本的检测也至关重要。综述了不同类型的单细胞蛋白定量检测方法,包括传统技术如流式细胞术、质谱流式细胞术、酶联免疫斑点检测法和毛细管电泳法,以及基于微流控芯片的新技术。比较了这些方法的原理、主要优缺点。与传统方法相比,微流控技术能更好地操纵单细胞,检测更灵敏,在单细胞蛋白检测领域具有更大潜力。Single-cell protein detection methods can detailedly explain life events,at the same time,it is crucial for detection of small amounts of samples.Different types of single cell protein quantitative detection methods are reviewed,including methods based on traditional techniques such as flow cytometry,mass cytometry,enzyme-linked immunospot assay and capillary electrophoresis,and novel technology based on microfluidic chips.The principles,main advantages and disadvantages of these methods are compared.Microfluidic technology can better manipulate single cells and detect more sensitively,so it has greater potential in single cell protein detection.

关 键 词：单细胞分析 蛋白检测 定量检测 微流控技术 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]