基于微流控芯片的乳腺癌肿瘤干细胞富集  

Breast cancer stem cells enrichment based on microfluidic chip

作　　者：王少熙[1,2] 阴玥 胡晨霞 WANG Shaoxi;YIN Yue;HU Chenxia(School of Microelectronics,Northwestern Polytechnical University,Xi'an 710072,China;Research & Development Institute of Northwestern Polytechnical University in Shenzhen,Shenzhen 518057,China;School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]西北工业大学微电子学院,陕西西安710072 [2]西北工业大学深圳研究院,广东深圳518057 [3]广州中医药大学中医学院,广东广州510006

出　　处：《传感器与微系统》2019年第10期73-75,共3页Transducer and Microsystem Technologies

基　　金：深圳基础研究基金资助项目(JCYJ2016042915311908);西安市科技计划项目(201805042YD20CG26)

摘　　要：为了克服传统方法低通量、低精度、低控制性和低效率的缺点,提出一种基于微流控技术研究肿瘤干细胞。基于二甲基硅氧烷,设计三层结构微流控芯片,结构包括细胞及培养液注入层、中间悬浮培养层和废液处理层。采用真空等离子氧化处理完成对准键合。芯片实验结果证明了微流控芯片的可行性和有效性。Aiming at the problems of low throughput,low precisim,poor controlling,low efficiency of the traditional experimental methods,a microfluidic chip technology is proposed to study on breast cancer stem cells.A microfluidic chip for breast cancer stem cells culturing in a suspension condition with three-layer structure is designed.Cell capturing and enrichment structure is set at the middle layer.Top layer and bottom layer are used to input stem cell and culturing medium respectively and output medium individually.Experimental results demonstrate the feasibility and effectiveness of the microfluidic chip.

关 键 词：乳腺癌肿瘤干细胞 微流控 富集 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]