磷掺杂碳量子点的制备及其在金丝桃苷检测中的应用  

Preparation of Phosphorus-doped Carbon Dots and Their Application in Hyperin Detection

作　　者：刘荔贞[1] 李海红 冯锋[1] 米智[1] LIU Li-zhen;LI Hai-hong;FENG Feng;MI Zhi(College of Chemistry and Chemical Engineering,Shanxi Datong University,Datong 037009,China;Schoolof Chemistry and Materials Science,Shanxi Normal University,Linfen 041004,China)

机构地区：[1]山西大同大学化学与环境工程学院,山西大同037009 [2]山西师范大学化学与材料科学学院,山西临汾041004

出　　处：《分析测试学报》2019年第10期1234-1239,共6页Journal of Instrumental Analysis

基　　金：大同市重点研发计划项目(2018014)

摘　　要：以五氧化二磷和柠檬酸为原料,采用自催化法快速制备了磷掺杂的碳量子点(P-CDs),通过透射电镜、红外光谱、X射线光电子能谱、紫外和荧光光谱对其进行表征。结果表明,P-CDs的粒径在5.0~10.2nm范围内,表面含有羟基、羧基和含磷官能团。基于金丝桃苷(HP)对P-CDs的荧光猝灭作用,建立了一种检测HP的新方法。该方法具有高的选择性和灵敏度。在优化条件下,HP对P-CDs的荧光猝灭率(IF0/IF)与HP的浓度在0.22~55μmol/L范围内呈良好的线性关系,检出限为78nmol/L。该方法被成功用于药品复方木鸡颗粒中HP的检测,回收率为93.3%~107%,相对标准偏差为1.5%~1.7%。In this paper,phosphorus-doped carbon dots(P-CDs)were prepared by a self-catalysis method with diphosphorus pentoxide and citric acid as raw materials.The P-CDs were characterized by transmission electron microscopy,Fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,UV-visible absorption and fluorescence spectrometry.Results showed that the sizes of P-CDs mainly ranged from 5.0 nm to 10.2 nm,and on the surfaces of which existed hydroxyl,carboxyl and phosphorous groups.A novel method was developed for the sensitive and selective detection of hyperin(HP)based on the phenomenon that HP could quench the fluorescence of P-CDs.Under the optimum conditions,a good linear relationship was obtained between the fluorescence quenching efficiency(IF0/IF)and the concentration of HP in range of 0.22-55μmol/L with a detection limit of 78 nmol/L.The method was successfully applied in the determination of HP in fufangmuji granules with recoveries of 93.3%-107%and relative standard deviation of 1.5%-1.7%.

关 键 词：磷掺杂碳量子点 制备 金丝桃苷 检测 荧光猝灭 

分 类 号：O657.3[理学—分析化学] O613.62[理学—化学]