肿瘤血管的高分辨光声定量成像  

High-Resolution Photoacoustic Quantitative Imaging of Tumor Vessels

作　　者：陈宁波 周慧超 赵煌旋[2] 王柏权 宋亮 刘成波[2] 张建辉[1] Chen Ningbo;Zhou Huichao;Zhao Huangxuan;Wang Boquan;Song Liang;Liu Chengbo;Zhang Jianhui(School of Mechanical and Electrical Engineering , Guangzhou University , Guangzhou , Guangdong 510006, China;Research Lab for Biomedical Optics and Molecular Imaging , Shenzheti Institutes of Advanced Technology ,Chinese Academy of Sciences, Shenzhe, Guangdong 518055, China;Department of Ultrasound , the Third Affiliated Hospital, Sun Yat-Sen University ,Guangzhou . Guangdo/ig 510630, China)

机构地区：[1]广州大学机械与电气工程学院,广东广州510006 [2]中国科学院深圳先进技术研究院生物医学光学与分子影像研究室,广东深圳518055 [3]中山大学附属第三医院超声科,广东广州510630

出　　处：《中国激光》2019年第9期219-228,共10页Chinese Journal of Lasers

基　　金：国家自然科学基金(91739117);深圳市科技计划(JCYJ20160608214524052);广州大学科研能力提升计划(2018A001)

摘　　要：利用微米级分辨率的高分辨光声显微成像系统,对小鼠耳部肿瘤早期血管的生长以及肿瘤对抗血管治疗药物的响应过程进行连续无标记的成像监测;提出将基于三维Hessian矩阵的血管提取算法应用于肿瘤血管的光声定量成像,以提高肿瘤血管的提取精度,并定量分析肿瘤血管的直径、密度、弯曲度等形态学信息变化。结果表明,高分辨光声定量成像在肿瘤及其他以血管变化为病理特征的疾病机制研究方面具有巨大的应用潜力。We uses a high-resolution (micrometer scale) photoacoustic microscopic imaging system to continuously monitor early-stage tumor angiogenesis in the ear of mice and its response to anti-angiogenesis therapy. Further, a three-dimensional Hessian-matrix-based vascular extraction algorithm is proposed for the quantitative photoacoustic imaging of tumor vessels to improve the tumor vessel extraction accuracy. Subsequently, the morphological changes in various parameters, such as the diameter, density, and tortuosity, of the tumor vessels are quantitatively analyzed. Furthermore, the potential of high-resolution photoacoustic quantitative imaging to study the pathological mechanisms of tumors and other diseases characterized by vascular changes is demonstrated in this study.

关 键 词：医用光学 光声成像 定量分析 血管提取 血管生成 生物医学 

分 类 号：Q631[生物学—生物物理学]