高效液相色谱法测定辣条中苯并[a]芘的含量  

Determination of Benzo[a]pyrene in Spicy Bar by High Performance Liquid Chromatography

作　　者：林海禄[1] 彭雪娇[1] 孙毅 向虹霖[1] LIN Hai-lu;PENG Xue-jiao;SUN Yi;XIANG Hong-lin(School of Chemistry,Biology and Material Science,East China University of Technology,Nanchang 330013,China)

机构地区：[1]东华理工大学化学生物与材料科学学院

出　　处：《东华理工大学学报（自然科学版）》2019年第3期291-294,共4页Journal of East China University of Technology(Natural Science)

基　　金：江西省教育厅科技项目(GJJ150587);江西省高等学校省级教改课题(JXJG-15-6-16,JXJG-17-37-4);江西省普通本科高校中青年教师发展计划访问学者专项基金项目(赣教办函[2016]169号)

摘　　要：使用C18反相色谱柱,高效液相色谱检测仪,甲醇与水的混合溶液作为流动相,比例为90∶10,流速2.0mL/min,检测波长260nm,进样量为20μL,紫外检测器对样品中苯并[a]芘的含量进行定量检测。检出限为36.04μg/L,辣条中苯并[a]芘的平均加标回收率为102.5%,实验相对标准偏差为1.20%(n=9)。方法步骤简单、溶剂与试样消耗少、样品分析时间短,具有良好的精密度以及高回收率,适合于对辣条中苯并[a]芘浓度的有效检测。In the present method,a Shimadzu SPD-15C HPLC equiped with a reverse-phase column was used,the mobile phase was isocratic flow of methanol-water(90∶10)at 2.0 mL/min,detecter wavelength was set at 260 nm,sample amount of sample solution was 20μL.By using reverse-phase HPLC with a ultraviolet detector,content assay of benzo[a]pyrene was conducted.The limit of detection was 36.04μg/L.In real sample assay,recovery rate of benzo[a]pyrene in vegetable oil used for making spicy gluten was 102.5%.Evetual RSD was 1.20%(n=9).The way has simple steps,little consumption of solvent and sample,short analysis time,good precision and high recovery rate,thus this method can be applied in content assay of benzo[a]pyrene in vegetable oil used for making spicy gluten.

关 键 词：反相高效液相色谱法 辣条 苯并[A]芘 

分 类 号：O657.7[理学—分析化学] TS201.6[理学—化学]