秀珍菇菌丝体无细胞滤液的应用：银纳米颗粒的生物合成及其抗菌活性  

Application of cell-free filtrate of Pleurotus geesteranus mycelium: biosynthesis of silver nanoparticles and their antimicrobial activity

作　　者：董丽娜 刘学瑞[1] 王慧杰 王磊[2] 徐慧[1] 周宇迪 DONG Lina;LIU Xuerui;WANG Huijie;WANG Lei;XU Hui;ZHOU Yudi(College of Chemistry and Materials Science, Ludong University, Yantai 264025, China;College of Life Sciences, Ludong University, Yantai 264025, China;Shanghai HuaEr Private Middle School, Shanghai 200122, China)

机构地区：[1]鲁东大学化学与材料科学学院,烟台264025 [2]鲁东大学生命科学学院,烟台264025 [3]上海民办华二初级中学,上海200122

出　　处：《辐射研究与辐射工艺学报》2019年第5期41-48,共8页Journal of Radiation Research and Radiation Processing

基　　金：山东省重点研发计划（公益类科技攻关）项目（2019GSF111047）资助~~

摘　　要：利用秀珍菇的无细胞滤液合成了胶体银纳米粒子(AgNPs),探讨其体外抗菌活性.采用紫外可见吸收光谱、X射线衍射、透射电子显微镜,和Zeta电位对AgNPs进行表征.选择鳗弧菌、溶藻弧菌、大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌,和铜绿假单胞菌为研究对象,采用琼脂扩散法检测AgNPs的抗菌活性.以大肠杆菌为指示菌,确定AgNPs的最低抑菌浓度(MIC)和最低杀菌浓度(MBC).得到分散良好的球形或近球形的AgNPs,粒度范围为4.5~18.2 nm.所得到的AgNPs可以有效地抑制各种细菌的增长,MIC和MBC分别为8.2 μg/mL和32.5 μg/mL.利用秀珍菇的无细胞滤液可实现AgNPs的可控合成,并且具有优良的抗菌活性、抑菌活性和广谱抗菌活性.This study evaluated the in vitro antibacterial activity of silver nanoparticles (AgNPs) synthesized from the cell-free filtrate of Pleurotus geesteranus. AgNPs were characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction, transmission electron microscopy, and zeta potential. The antibacterial activity of the AgNPs was evaluated by the agar diffusion method against Vibrio anguillaru, Vibrio alginolyticus, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. Escherichia coli was selected as the index bacteria to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the AgNPs. Well-dispersed spherical or nearly spherical AgNPs were obtained with particle sizes ranging from 4.5 to 18.2 nm. The acquired AgNPs effectively inhibited the growth of various bacteria. The MIC and MBC were 8.2 μg/mL and 32.5 μg/mL, respectively. The controllable synthesis of AgNPs achieved using the cell-free filtrate of P. geesteranus produced AgNPs that exhibited excellent broad-spectrum antibacterial activity.

关 键 词：胶体银粒子 生物合成 秀珍菇 抗菌活性 

分 类 号：TL13[核科学技术—核能科学]