HPLC-MS/MS法测定人血清中异帕米星的浓度  

Determination of Isepamicin in Human Serum by HPLC-MS/MS

作　　者：林志燕[1] 黄晓会[1] 杨萍[1] 陆晓彤[1] Lin Zhiyan;Huang Xiaohui;Yang Ping;Lu Xiaotong(Department of Pharmacy, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China)

机构地区：[1]上海交通大学医学院附属新华医院药学部

出　　处：《中国药师》2019年第10期1918-1920,共3页China Pharmacist

基　　金：国家自然科学基金青年项目(编号:81603099);上海市卫生和计划生育委员会中医药开发研究基金项目(编号:2014XP001A);上海市卫生局中医药科研基金项目(编号:2012G003A)

摘　　要：目的:建立测定人血清中异帕米星的高效液相色谱-串联质谱(HPLC-MS/MS)方法。方法:色谱柱为Agilent Poroshell120 HILIC-Z柱(100 mm×2. 1 mm,2. 7μm),流动相为水溶液(100 mmol·L^-1醋酸铵+1%甲酸)-乙腈(1%甲酸),梯度洗脱。以阿米卡星为内标,电喷雾离子源,多反应离子监测模式,正离子模式检测。异帕米星定量离子通道为570. 2/411. 4,定性离子通道为570. 2/250. 3。结果:异帕米星在0. 78~25. 00μg·ml^-1范围内线性关系良好(r=0. 998 7),回收率为93. 22%~101. 96%。结论:本方法简单、快速、准确,适用于临床患者人血清异帕米星浓度分析测定。Objective: To establish a liquid chromatography-tandem mass spectrometry( LC-MS/MS) method for the determination of isepamicin in human serum. Methods: The analysis was carried out on an Agilent Poroshell120 HILIC-Z( 100 mm × 2. 1 mm,2. 7 μm) column with gradient elution. The mobile phase was H2 O( containing 1% formic acid and 100 mmol·L-1 ammonium acetate) and acetonitrile( containing 1% formic acid). The determination was performed on a triple quadrupole tandem mass spectrometer by multiple reactions monitoring( MRM) mode via electrospray ionization( ESI) source. The quantitative ion transition was570. 2/411. 4 and the qualitative ion transition was 570. 2/250. 3. Amikacin was used as the internal standard( IS). Results: The calibration curve was linear over the range from 0. 78 μg·ml-1 to 25. 00 μg·ml-1 with a correlation coefficient of 0. 998 7. The recovery was 93. 22%-101. 96%. Conclusion: The method is simple and accurate,and suitable for the determination of isepamicin in human serum.

关 键 词：异帕米星 高效液相色谱-串联质谱 治疗药物浓度监测 

分 类 号：R969.1[医药卫生—药理学]