piR-hsa-1282在脂多糖诱导的293T细胞急性损伤中的作用  

The role of piR-hsa-1282 in lipopolysaccharide-induced 293T cells injury

作　　者：李杨 王颖 王妍[1,2] 施鑫钰 陈瑾 钱晖 LI Yang;WANG Ying;WANG Yan;SHI Xin-yu;CHEN Jin;QIAN Hui(Zhenjiang Key Laboratory of High Techonology Research on Exosomes Foundation and Transformation Application;School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China)

机构地区：[1]镇江市外泌体基础与转化应用高技术研究重点实验室 [2]江苏大学医学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2019年第5期386-389,393,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81871496);江苏大学学生科研项目(17A471)

摘　　要：目的:探讨piR-hsa-1282在脂多糖诱导的293T细胞损伤中的作用。方法:体外建立脂多糖诱导的293T细胞损伤模型,实时荧光定量PCR(qRT-PCR)检测piR-hsa-1282的表达水平,ELISA检测IL-6的分泌改变。蛋白质印迹法分析抑制剂敲减piR-hsa-1282后,293T细胞中凋亡蛋白cleaved-caspase 3、cleaved-caspase 9及炎症蛋白IL-1β表达改变。结果:脂多糖刺激293T细胞24 h后,细胞活力减弱,凋亡蛋白cleaved-caspase 3表达增强,炎性因子IL-6分泌增多,并且piR-hsa-1282表达也明显上调。与对照组相比,piR-hsa-1282敲减后293T细胞中cleaved-caspase 3、cleaved-caspase 9及IL-1β蛋白表达降低。结论:piR-hsa-1282可能通过调节细胞凋亡和炎性反应参与脂多糖诱导的293T细胞急性损伤。Objective:To investigate the role of piR-hsa-1282 in lipopolysaccharide(LPS)-induced 293T cells injury.Methods:A 24 h LPS-treated 293T cells was established as the acute injury model.qRT-PCR was used for determing the expression level of piR-hsa-1282.ELISA was applied for detection of IL-6 expression.Western Bloting was used to detect the expression of cleaved-caspase 3,cleaved-caspase 9 and IL-1βafter piR-hsa-1282 inhibition.Results:With stimulation of LPS for 24 h,cell viability was reduced and the expression levels of cleaved-caspase 3 and IL-6 were up-regulated.Meanwhile,there was a higher level of piR-hsa-1282.Compared with the control group,piR-hsa-1282 inhibition led to the down-regulation of cleaved-caspase 3,cleaved-caspase 9 and IL-1β.Conclusion:piR-hsa-1282 could be involved in LPS-induced injury through regulation of apoptosis and inflammatory response.

关 键 词：脂多糖 293T细胞 细胞损伤 piR-hsa-1282 凋亡 炎症 基因敲减 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]