知柏地黄汤治疗萎缩性阴道炎的疗效及对调节大鼠内分泌功能、改善生殖道衰老的作用  

Effect of Zhibai Dihuang Decoction on Atrophic Vaginitis and Its Effect on Regulating Endocrine Function and Improving Reproductive Tract Senility in Rats

作　　者：张媛媛[1] 张旋[1] 宋丽华[1] 周京晶[1,2] ZHANG Yuan-yuan;ZHANG Xuan;SONG Li-hua;ZHOU Jing-jing(Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Xianyang 712000)

机构地区：[1]陕西中医药大学第二附属医院,咸阳712000 [2]安康市中医医院妇科,安康725000

出　　处：《河北中医药学报》2019年第5期11-14,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：陕西省科技厅重点研发计划项目:No.2017SF-323

摘　　要：目的:研究知柏地黄汤治疗萎缩性阴道炎的疗效及对大鼠内分泌功能、生殖道衰老的影响。方法:选取10月龄SD雌性大鼠88只作为本次研究对象,将其随机分为对照1组(22只),对照2组(22只)、西药组(22只)以及中药组(22只)。对照1组行手术但不切除卵巢,对照2组、西药组以及中药组大鼠经卵巢切除手术建立萎缩性阴道炎动物模型。对照1组和对照2组大鼠均予以生理盐水灌胃,西药组大鼠予以戊酸雌二醇灌胃,中药组大鼠予以知柏地黄汤灌胃,所有大鼠均连续用药30 d。比较4组大鼠阴道pH值、子宫及阴道重量指数、血清卵泡生成激素、雌二醇、黄体生成激素、丙二醛、活性氧簇、超氧化物歧化酶水平。结果:对照2组、西药组大鼠阴道pH值显著高于对照1组,差异有显著性( P <0.05);对照2组、西药组及中药组大鼠血清卵泡生成激素、黄体生成激素、丙二醛、活性氧簇均显著高于对照1组,子宫及阴道重量指数、雌二醇、超氧化物歧化酶均显著低于对照1组,差异有显著性( P <0.05);西药组及中药组大鼠阴道pH值、血清卵泡生成激素、黄体生成激素、丙二醛、活性氧簇均显著低于对照2组,子宫及阴道重量指数、雌二醇、超氧化物歧化酶均显著高于对照2组,差异有显著性( P <0.05);西药组与中药组大鼠血清卵泡生成激素、雌二醇、黄体生成激素比较差异无显著性( P >0.05);中药组大鼠阴道pH值、血清丙二醛、活性氧簇显著低于西药组,子宫及阴道重量指数、超氧化物歧化酶均显著高于西药组,差异有显著性( P <0.05)。结论:知柏地黄汤在治疗萎缩性阴道炎具有良好疗效,能够有效调节大鼠内分泌功能,改善大鼠生殖道衰老情况。Objective: To study the therapeutic effect of Zhibai Dihuang Decoction on atrophic vaginitis and its effects on endocrine function and aging of reproductive tract in rats. Methods: Eighty-eight female SD rats aged 10 months were randomly divided into control group 1 (22 rats), control group 2 (22 rats), western medicine group (22 rats) and Chinese medicine group (22 rats).Control group 1 underwent operation without ovariectomy, control group 2, western medicine group and Chinese medicine group were ovariectomized to establish atrophic vaginitis animal model.Rats in control group 1 and 2 were given normal saline, rats in western medicine group were given estradiol valerate,and rats in Chinese medicine group were given Zhibai Dihuang decoction. All rats were administrated for 30 days. Vaginal pH, body mass index of uterus and vagina, serum follicle-forming hormone, estradiol, luteinizing hormone, malondialdehyde, reactive oxygen species (ROS) and superoxide dismutase (SOD) levels were compared in the four groups. Results: The vaginal pH value of rats in control group 2 and Western medicine group was significantly higher than that in control group 1 ( P <0.05 );the serum follicle-forming hormone, luteinizing hormone, malondialdehyde and ROS in control group 2, western medicine group and Chinese medicine group were significantly higher than those in control group 1, and the body mass index, estradiol and superoxide dismutase were significantly lower than those in control group 1 ( P <0.05).The vaginal pH value, serum follicle-forming hormone, luteinizing hormone, malondialdehyde and ROS were significantly lower in the western medicine group and Chinese medicine group than those in the control group.The body mass index, estradiol and SOD were significantly higher in western medicine group and the traditional Chinese medicine group than those in the control group ( P <0.05).There was no significant difference among hormones, estradiol and luteinizing hormone between western and Chinese medicine groups ( P >0.05).Vagina

关 键 词：知柏地黄汤 萎缩性阴道炎 带下病 阴痒 大鼠 内分泌功能 生殖道衰老 

分 类 号：R285.6[医药卫生—中药学]