海南龙血树4个DcTIFY10转录因子的鉴定和表达分析  

Identification and Expression Analysis of Four Transcription Factor DcTIFY10 in Dracaena cambodiana

作　　者：丁旭坡 马纳纳 庞宽壮 蔡彩虹[1,2] 戴好富[1,2] 梅文莉[1,2] Ding Xupo;Ma Nana;Pang Kuanzhuang;Cai Caihong;Dai Haofu;Mei Wenli(Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture and Rural Affairs,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,570228)

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业农村部热带作物生物学与遗传资源利用重点实验室,海口571101 [2]中国热带农业科学院热带生物技术研究所,海南省黎药天然产物研究与利用重点实验室,海口571101 [3]海南大学热带农林学院,海口570228

出　　处：《分子植物育种》2019年第19期6287-6294,共8页Molecular Plant Breeding

基　　金：海南省重点研发计划(ZDYF2018226);国家自然科学基金(81803663);中国热带农业科学院基本科研业务费专项资金(17CXTD-15)共同资助

摘　　要：TIFY家族是陆生植物特有的基因家族,而TIFY10/JAZ则是植物茉莉酸信号转导的关键调控因子。本研究在海南龙血树(Dracaena cambodiana)转录组数据基础上,利用生物信息学方法首次鉴定了4个编码177~246个氨基酸的海南龙血树DcTIFY10基因,4个DcTIFY10蛋白具有TIFY10的特征性ZIM和Jas结构域,与水稻、拟南芥的TIFY10具有较高的同源性,与水稻OsTIFY10基因家族具有较近的亲缘关系,4个DcTIFY10蛋白均无跨膜结构域,分别包含20~30个磷酸化位点,蛋白二级结构主要有α螺旋和无规则卷曲为主;4个DcTIFY10基因在海南龙血树根或者茎中的表达量最高,在血竭诱导生成过程中持续上调表达,与血竭的生成部位和形成时间呈现出一致性,说明其在血竭生成过程中发挥着重要的作用,此结果为进一步研究茉莉酸信号途径在海南龙血树生长发育和血竭形成过程中的作用提供了技术支持。TIFY family is the special gene family in terrestrial plants and the TIFY10/JAZ is the key regulatory factor in plant jasmonates signal transduction.In this study,four DcTIFY10 genes encoding 177~246 amino acids were identified with bioinformatic and phylogenetic analysis in Dracaena cambodiana based on its transcriptome data.These four DcTIFY10 protein contained ZIM and Jas as the characteristic structural domains of TIFY,and there were higher homology with the TIFY10 among D.cambodiana,Arabidopsis thaliana and Oryza sativa,and there were close genetic relationship between TIFY10 from D.cambodiana and O.sativa than A.thaliana.The physicochemical property analysis showed that four DcTIFY10 proteins included 20~30 phosphorylation sites without transmembrane domain and their secondary structure were constituted withα-helix and random coil principally.The tissues special and induction expression profiles of four DcTIFY10 in D.cambodiana with real time fluorescence quantitative PCR demonstrated that their relative expression in root or stem was higher than leaf or flower,and the relative expression of four DcTIFY10 continuously increased in stem of D.cambodiana suggested that there was important function of DcTIFY10 during dragon blood formation.This study provided important research basis with theory and technology for the functional study of JA signaling in growth,development and dragon blood formation of D.cambodiana.

关 键 词：海南龙血树(Dracaena cambodiana) TIFY10 转录因子 生物信息学 表达分析 

分 类 号：S567.19[农业科学—中草药栽培]