自发性糖尿病食蟹猴的诊断及其骨微结构的改变  

Diagnosis of and bone microstructure in spontaneous diabetic cynomolgus monkeys

作　　者：贾欢欢[1] 罗晓玲 高洪彬 梁十 罗挺[1] 陈结文 何积存 黄庆宇 李永锋 卢丽 陈梅丽[1] JIA Huanhuan;LUO Xiaoling;GAO Hongbin;LIANG Shi;LUO Ting;CHEN Jiewen;HE Jicun;Huang Qingyu;LI Yongfeng;LU Li;CHEN Meili(Guangdong Laboratory Animals Monitoring Institute,Key Laboratory of Guangdong Laboratory Animals,Guangzhou 510663,China;School of Life Sciences and Biopharmacy,Guangdong Pharmaceutical University,Guangzhou 510006;Guangzhou Blooming-Spring Biological Research Institute Co,Ltd.,Guangzhou 510940)

机构地区：[1]广东省实验动物监测所/广东省实验动物重点实验室,广州510663 [2]广东药科大学生命科学与生物制药学院,广州510006 [3]广州春盛生物研究院有限公司,广州510940

出　　处：《中国实验动物学报》2019年第5期626-630,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：广东省省级科技计划项目(2017A070701003,2017B020231001,2019A030317009)~~

摘　　要：目的,对自发性糖尿病食蟹猴进行诊断,并分析其骨微结构的变化,以期建立自发性糖尿病合并骨质疏松动物模型。方法选取13~20岁的自发性糖尿病食蟹猴4只,以年龄相近的动物作为对照;利用快速血糖仪检测空腹血糖及糖耐量,生化仪检测血清中相关生化指标,以此对自发性糖尿病食蟹猴进行诊断;利用Micro-CT测量股骨下段和胫骨上段骨结构,分析自发性糖尿病食蟹猴骨微结构的变化。结果自发性糖尿病组食蟹猴的空腹血糖大于8.0 mmol/L,糖耐量试验120 min时血糖大于10.0 mmol/L,且TG、LDL-C与对照组比较显著升高,HDL-C及Crea显著低于对照组,TC及BUN无明显差异,可确诊为自发糖尿病动物;Micro-CT结果显示自发性糖尿病食蟹猴骨微结构破坏明显,呈骨质疏松表现,且股骨下段和胫骨上段松质骨骨小梁面积、骨小梁体积及骨矿含量均显著低于对照组,而股骨下段和胫骨上段皮质骨两组间比较无明显差异。结论自发性糖尿病食蟹猴与人类糖尿病患者有着相似的临床特征,可作为一种理想的糖尿病合并骨质疏松动物模型。Objective To diagnose the spontaneous diabetic cynomolgus monkey and examine their bone microstructure,and establish an animal model of spontaneous diabetic osteoporosis.Methods Four spontaneous diabetic cynomolgus monkeys (13 to20 years old) were included.Animals of similar ages were used as a control group.Fasting blood glucose and glucose tolerance were measured using a fast blood glucose meter.TC,LDL-C,HDL-C,TG,Crea,and BUN were measured to make the diagnosis of spontaneous diabetic cynomolgus monkeys. Micro-CT was used to measure the bone structure of distal femur and upper tibia.Results In the spontaneous diabetic group,the fasting blood glucose was higher than 8.0 mmol/L, and the blood glucose was higher than10.0 mmol/L at120 min of the glucose tolerance test.TG and LDL-C of the spontaneous diabetic cynomolgus monkeys were significantly higher than the control group,while HDL-C and Crea were significantly lower than the control group ( P<0.05).There was no significant difference between TC and BUN,which could be diagnosed as spontaneously spontaneous diabetic cynomolgus monkeys.Micro- CT results showed that the distal femur and upper tibia of the spontaneous diabetic cynomolgus monkeys were damaged indicating osteoporosis.Moreover,the trabecular area, trabecular volume and bone mineral content of cancellous bone in the distal femur and upper tibia of the spontaneous diabetic cynomolgus monkeys were significantly lower than the control group.There were no significant differences in the cortical bone of the distal femur and upper tibia between the two groups.Conclusions The spontaneous diabetic cynomolgus monkeys have similar clinical characteristics with human patients,and may be used as an excellent animal model in studies of diabetic osteoporosis.

关 键 词：食蟹猴 糖尿病合并骨质疏松 动物模型 糖耐量 骨结构 

分 类 号：Q95-33[生物学—动物学]