甘西鼠尾草对高原肺动脉高压大鼠的干预作用及可能机制  

Study on the intervention effects and possible mechanisms of Salvia przewalskii Maxim.on the high-altitude pulmonary hypertension rats

作　　者：王亚峰[1] 王爱霞 王生彪 朵德龙[1] 严英俊 李茜 WANG Yafeng;WANG Aixia;WANG Shengbiao;Duo Delong;YAN Yingjun;Li Qian(QingHai Province People’s Hospital,Xining,Qinghai 810007)

机构地区：[1]青海省人民医院

出　　处：《中国高原医学与生物学杂志》2019年第3期158-162,共5页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省科技厅基础研究项目(2018-ZJ-786)

摘　　要：目的探究甘西鼠尾草(SalviaprzewalskiiMaxim.SPM)对高原肺动脉高压(HAPH)大鼠的干预作用及可能机制。方法采用实地海拔环境,以平均肺动脉压(mPAP)和右心室肥大指数(RVHI)为评价指标,探究SPM对高原(4260m)环境处理4w大鼠肺动脉高压的干预作用。同时通过RT-PCR法测定大鼠肺组织中的缺氧诱导因子(HIF-1α)、核因子-κB(NF-κB)和单核细胞趋化因子(MCP1)的mRNA表达水平探究其干预作用的可能机制。结果(1)缺氧组即高原连续处理4w大鼠的mPAP及RVHI均较对照组明显升高(P<0.05),SPM剂量组的mPAP和RVHI均呈剂量依赖性降低,其中除低剂量组(0.5g·kg^-1)的mPAP较缺氧组无明显降低外(P>0.05),中(1g·kg^-1)、高(2g·kg^-1)剂量组的mPAP及各剂量组的RVHI均较缺氧组明显降低(P<0.05)。(2)缺氧组大鼠肺组织中HIF-1α、NF-κB和MCP1的mRNA表达水平较对照组明显升高(P<0.05),SPM剂量组的HIF-1α、NF-κB和MCP1的mRNA表达水平均呈剂量依赖性降低,其中除低剂量组(0.5g·kg^-1)的HIF-1αmRNA表达水平较缺氧组无明显降低(P>0.05)外,中(1g·kg^-1)、高(2g·kg^-1)剂量组的HIF-1αmRNA表达水平及各剂量组的NF-κB和MCP1的mRNA表达水平均较缺氧组明显降低(P<0.05)。结论SPM对大鼠HAPH具有一定的预防和改善作用,其机制可能与下调HAPH大鼠肺组织中HIF-1α、NF-κB和MCP1的mRNA表达有关。Objective To explore the intervention effects and possible mechanisms of Salvia przewalskii Maxim.(SPM)on high-altitude pulmonary hypertension(HAPH)rats.Methods Under the field of altitude environment and taking the mean pulmonary hypertension(mPAP)and right ventricular hypertrophy index(RVHI)as the evaluation indexes,the research of investigating the effects of SPM on HAPH rats which were treated at high attitude(4260m)for 4 weeks was conducted.Meanwhile,the mRNA expression levels of HIF-1α,NF-κB and MCP1 in lung tissues were determined by RT-PCR method,so as to explore the relevant mechanisms of SPM.Results(1)Compared with the control group,the mPAP and RVHI of the hypoxia group in which the rats were continuously treated for 4 weeks were significantly increased(P<0.05).And the mPAP and RVHI of the SPM groups were decreased in a dose-dependent manner.Excepting the mPAP in the low-dose group(0.5g·kg^-1)did not decreased significantly compared with the hypoxia group(P>0.05),the mPAP in the medium-dose(1g·kg^-1)and high-dose(2g·kg^-1)group and the RVHI in each SPM dose group were all significantly reduced compared with the hypoxia group(P<0.05).(2)Compared with the control group,the mRNA expression level of the HIF-1α,NF-κB and MCP1 in the hypoxia group were all significantly increased(P<0.05).And the mRNA expression level of the HIF-1α,NF-κB and MCP1 of the SPM groups were decreased in a dose-dependent manner.Excepting the mRNA expression level of the HIF-1αin the low-dose group(0.5g·kg^-1)did not decreased significantly compared with the hypoxia group(P>0.05),the mRNA expression level of the HIF-1αin the medium-dose(1g·kg^-1)and high-dose(2g·kg^-1)group and the mRNA expression level of NF-κB and MCP1 in each SPM dose group were all significantly decreased compared with the hypoxia group(P<0.05).Conclusion The SPM can prevent and improve HAPH in rats,and the possible mechanism may be related to the down-regulation of mRNA expression level of HIF-1α,NF-κB and MCP1 in the lung tissue.

关 键 词：甘西鼠尾草 高原 肺动脉高压 缺氧诱导因子 核因子-ΚB 单核细胞趋化因子 

分 类 号：R594.3[医药卫生—内科学]