低氧预处理和七氟醚预处理对短暂性全脑缺血成年大鼠的保护作用及作用机制  

The protective effect and mechanism of hypoxic preconditioning and sevoflurane preconditioning on adult rats with transient cerebral ischemia

作　　者：王道合 赵秀丽[2] 拜承萍[2] WANG Daohe;ZHAO Xiuli;BAI Chengping(Qinghai University Medical College;Affiliated Hospital of Qinghai University)

机构地区：[1]青海大学医学院 [2]青海大学附属医院

出　　处：《中国高原医学与生物学杂志》2019年第3期163-167,共5页Journal of Chinese High Altitude Medicine & Biology

摘　　要：目的了解低氧预处理和七氟醚预处理对短暂性全脑缺血(tGCI)成年大鼠的保护作用及作用机制。方法将成年健康雄性大鼠分成全脑缺血再灌注损伤组(I/R组)、低氧预处理组(Hyp组)、七氟醚预处理组(Sev组)。采用FJB染色法观察各组大鼠海马CA1区神经元坏死情况、Qpcr法检测海马组织中Jun-BmRAN的相对表达量。并对上述结果行相关分析。结果(1)与I/R组比较,在T1、T2、T3点,各组FJB阳性细胞明显减少(P<0.01)。(2)与I/R组比较,在T1、T2、T3点,各组海马Jun-BmRAN表达量均显著较少(P<0.01)。结论低氧预处理、七氟醚预处理对短暂性全脑缺血成年大鼠具有保护性作用,可能的作用机制是通过对Jun-BmRAN表达的影响发挥脑保护作用。Objective To investigate the protective effect and mechanism of hypoxic preconditioning and sevoflurane preconditioning on adult rats with transient global cerebral ischemia(tGCI).Methods Adult healthy male rats were divided into whole brain ischemia reperfusion injury group(I/R group),hypoxic preconditioning group(Hyp group),and sevoflurane preconditioning group(Sev group).FJB staining was used to observe the neuronal necrosis in CA1 area of hippocampus in each group of rats.The relative expression of Jun-B mRAN in hippocampus was detected by Qpcr.The above results were analyzed with correlation analysis.Results(1)compared with the I/R group,FJB positive cells were significantly reduced in T1,T2 and T3(P<0.01).(2)compared with the I/R group,the expression levels of jun-b mRAN in the hippocampus of each group were significantly lower at T1,T2 and T3(P<0.01).Conclusion hypoxic preconditioning and sevoflurane preconditioning have protective effects on adult rats with transient cerebral ischemia,and the possible mechanism is to exert brain protective effects through the effect on the expression of Jun-B mRAN.

关 键 词：低氧 七氟醚 预处理 即刻早基因 全脑缺血再灌注 保护 

分 类 号：R614[医药卫生—麻醉学]