基于16S rRNA基因白眉长臂猿属物种鉴定研究  

Molecular Identification of Hoolock Gibbon Species Based on the 16s rRNA Gene

作　　者：胡桓嘉 滕萍 李媛 番玉买 李宏刚 王勒端 段玉宝[2,3] HU Huanjia;TENG Ping;LI Yuan;FAN Yumai;LI Honggang;WANG Leduan;DUAN Yubao(Dehong State Wildlife Rescue Center,Dehong,678400,China;Key Laboratory for Conserving Wildlife with Small Populations in Yunnan,Southwest Forestry University,Kunming,650224,China;Faculty of Biodiversity Conservation,Southwest Forestry University,Kunming,650224,China)

机构地区：[1]德宏州野生动物收容救护中心,德宏678400 [2]西南林业大学,云南省高校极小种群野生动物保育重点实验室,昆明650224 [3]西南林业大学生物多样性保护学院,昆明650224

出　　处：《野生动物学报》2019年第4期890-894,共5页CHINESE JOURNAL OF WILDLIFE

基　　金：国家林业局野生动植物保护管理项目(2018);珍稀濒危物种野外救护与人工繁育(2130211)

摘　　要：分子生物学作为重要的辅助手段是物种分类和物证鉴定常用的方法之一。高黎贡白眉长臂猿(Hoolock tianxing)于2017年从东白眉长臂猿(H.leuconedys)中分离出来,独立成种,但两者幼体的形态学特征并不明显,形态鉴别较难。本研究基于16S rRNA基因对3个白眉长臂猿属物种(样品分别标记为B1、B2、B3)进行物种鉴定。BLAST比对结果显示,B1与东白眉长臂猿(GenBank:KY250071.1)的同源性为100%,B2与天行长臂猿(KY250070.1)的同源性为100%,B3与东白眉长臂猿(KY250074.1)的同源性为99.61%。系统发育树分析显示:B1、B3与东白眉长臂猿的聚为一支,B2与4条天行长臂猿的序列聚为一支,支持率达100%。综上,B1、B3为东白眉长臂猿,B2为天行长臂猿。本次研究为16S rRNA基因在灵长类物种鉴定和野生动物司法鉴定上提供参考。Molecular biology is an important auxiliary and a common method for species classification and physical evidence identification. Hoolock tianxing was isolated from H.leuconedys in 2017 and became a species.But the juveniles of both species share morphological characteristics that are so similar that they preclude identification to species.In this study,we identified three samples (labeled B1,B2,B3) of Hoolock based on the 16S rRNA gene.BLAST results showed that the identities between B1 and H.leuconedys (GenBank:KY250071.1) were 100%,B2 and H.tianxing (KY250070.1) were 100%,and B3 and H.leuconedys (KY250074.1) were 99.61%.Phylogenetic tree analysis showed that the sequences of B1,B3 and H.leuconedys were clustered as one branch,and B2 and H.tianxing were clustered as one branch with the support rate reaching 100%.In summary,B1 and B3 both proved to be H.leuconedy ,and B2 was identified as H.tianxing .This study provides reference for the 16S rRNA gene in primate species identification and wildlife forensic identification.

关 键 词：东白眉长臂猿属 高黎贡白眉长臂猿 16S RRNA基因 物种鉴别 

分 类 号：Q75[生物学—分子生物学]