16S rRNA测序法检测仓鼠颊囊内细菌种类的研究  

Research on Bacteria Species in Hamster Cheek Pouches by Use of 16S rRNA Sequencing

作　　者：陈璟恒 张芙蕊 李和平[1] CHEN Jingheng;ZHANG Furui;LI Heping(College of Wildlife and Protected Area,Northeast Forestry University,Harbin,150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院

出　　处：《野生动物学报》2019年第4期1015-1017,共3页CHINESE JOURNAL OF WILDLIFE

基　　金：东北林业大学国家级大学生创新训练项目(201710225092)

摘　　要：针对仓鼠颊囊中微生物栖居情况尚不清楚的问题,以确定仓鼠颊囊内细菌种类对其颊囊内食物的消化生理作用为目的,本研究采用局部解剖法对仓鼠的颊囊进行解剖,并从仓鼠颊囊中提取16S rRNA,进行仓鼠颊囊中的微生物物种分类和丰富度分析。结果表明,颊囊中含有拟杆菌属(Bacteroides)、色杆菌属(Chromobacterium)、乳杆菌(Lactobacillus)、德克斯氏菌属(Derxia)、葡萄球菌属(Staphylococcus)、明串珠菌属(Leuconostoc)等微生物,其中乳杆菌等对仓鼠贮存在颊囊中的食物具有一定促进消化的作用。Species of microorganisms inhabiting the cheek pouches of hamsters are not well documented.In this study,we aimed to determine the digestive physiological effect of the bacterial species on hamster food.We dissected hamster cheek pouches and sequenced the 16S rRNA from them to identify the bacterial species and analyze species richness.The cheek pouches contained Bacteroides , Chromobacterium,Lactobacillus,Derxia,Staphylococcus ,and Leuconostoc .Among these microorganisms, Lactobacillus has a certain effect on promoting digestion of the food stored in the cheek pouches of the hamster.

关 键 词：仓鼠 颊囊 细菌 

分 类 号：Q93[生物学—微生物学]