细胞适应性流感病毒高产株的制备研究  

Advances in preparation of cell-adapted high-yield strains from influenza virus

作　　者：谢莹 李长贵[1] XIE Ying;LI Chang-gui(National Institutes for Food and Drug Control, Division of Respiratory Virus Vaccines, Beijing102629, China)

机构地区：[1]中国食品药品检定研究院呼吸道病毒疫苗室

出　　处：《微生物学免疫学进展》2019年第5期65-69,共5页Progress In Microbiology and Immunology

摘　　要：与鸡胚培养制备的流感疫苗相比,细胞制备的疫苗具有免疫原性好、生产不受鸡胚限制等优点。但目前流感病毒株在细胞上产量较低,成为疫苗生产的主要限制因素。现就用于制备细胞适应性高产株主要的3种方法,即连续传代、随机突变构建病毒突变体和病毒重配的研究进展,以及突变位点对病毒增殖的影响作一概述。In comparison with influenza vaccine derived from chicken embryo, influenza vaccine based on cell culture has the advantages with a good immunogenicity, and not restricted to the production by chicken embryo.However, currently most influenza virus strains do not grow efficiently on cells, which become the main limiting factor in the vaccine production.In this article, we review the current progress in search for various methods for preparing high growth strains on cells, including continuous passages and plaque purification, construction of viral mutations by random mutagenesis, and co-infection or reverse genetics for the viruses, as well as the influencing mutations to the virus yield.

关 键 词：细胞适应性 流感病毒 高产株 

分 类 号：R373.13[医药卫生—病原生物学]