温针灸对骨关节炎大鼠软骨组织Clock、Bmal1蛋白的影响  

Effects of Warming Acupuncture on Cartilage Tissue Protein Expressions of Clock and Bmal1 in Osteoarthritis Rats

作　　者：蔡国伟[1] 李柏村 李佳 李静[1] CAI Guo-wei;LI Bo-cun;LI Jia;LI Jing(Department of Acupuncture,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan (430022);College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion,Wuhan (430061))

机构地区：[1]华中科技大学同济医学院附属协和医院针灸科,武汉430022 [2]湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心,武汉430061

出　　处：《中国中西医结合杂志》2019年第10期1225-1229,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81774410);湖北省卫健委项目(No.ZY2019M041)

摘　　要：目的探讨温针灸对骨关节炎(OA)大鼠软骨组织Clock、Bmal1蛋白表达的影响。方法 60只3月龄SPF级雄性SD大鼠,按随机数字表法分为对照组、模型组、针刺组、温针灸组,每组15只。以手术方法制备OA大鼠,温针灸组于造模第1天取足三里、犊鼻和内膝眼穴温针灸治疗,采用手针及悬灸法,15 min/次,1次/天,共21天。针刺组采用针刺方法同温针灸组,不用艾灸。对照组大鼠自由饮水,正常饲养。所有大鼠于相应处理结束后,采用排水法测大鼠膝关节部体积,HE染色观察软骨组织病理结构,Western Blot检测软骨组织Clock、Bmal1 蛋白表达。结果与对照组比较,模型组大鼠右膝关节肿胀明显(P<0.01);模型组大鼠软骨组织Clock、Bmal1蛋白表达明显降低(P<0.01);与模型组比较,针刺和温针灸均能显著改善大鼠右膝关节肿胀程度(P<0.05),提高OA大鼠软骨组织中Clock、Bmal1蛋白表达水平(P<0.05),温针灸组较针刺组改善更为明显(P<0.05)。结论相对于针刺,温针灸更能有效调节OA大鼠关节炎性损伤状况,促进OA大鼠软骨组织中Clock、Bmal1蛋白表达,从而调节软骨细胞生物钟,抑制了软骨细胞骨架破坏的进程,减缓膝关节的关节退变。Objective To observe the effects of warming acupuncture on protein expressions of Clock and Bmal1 in cartilage tissue of rats with osteoarthritis(OA). Methods Sixty 3-month-old male Sprague-Dawley(SD) rats were randomly divided into four groups by random number table, i.e., the control group, the model group, the acupuncture group, and the warming acupuncture group, 15 in each group. OA rat model were prepared by surgery. Rats in warming acupuncture group were needled at Zusanli(ST36), Dubi(ST35), Neixiyan(EX-LE4) on day 1 of modeling, 15 min per time, once a day and totally for 21 days. Rats in acupuncture group were treated with the same acupuncture method as the warming acupuncture group without moxibustion. Rats in the control group were fed normally and free access to normal water. The volume of knee joints was detected using drainage. The pathological structures of cartilage tissue were observed by HE staining. The expressions of Clock and Bmal1 protein in cartilage tissue were detected by Western Blot. Results Compared with the control group, the right knee joint was obviously swollen(P<0.01), the expressions of Clock and Bmal1 protein in cartilage tissue significantly decreased in the model group(P<0.05). Compared with the model group, the swollen right knee joint was obviously ameliorated(P<0.05), the expressions of Clock and Bmal1 protein in cartilage tissue significantly elevated in the acupuncture group and the warming acupuncture groups(P<0.05). Better effects were obtained in the warming acupuncture group(P<0.05). Conclusion Compared with acupuncture, warming acupuncture regulated inflammatory injury of OA rats more effectively, promoted expressions of Clock and Bmal1 proteins in cartilage tissue of OA rats, thus regulating the circadian clock of chondrocytes, inhibiting the process of cartilage cytoskeleton destruction, and relieving the deterioration of knee OA.

关 键 词：温针灸 足三里 骨关节炎 Clock蛋白 Bmal1蛋白 

分 类 号：R245[医药卫生—针灸推拿学]