补肾活血方调控兔激素性股骨头坏死APN-AMPK信号通路的实验研究  

Bushen Huoxue Recipe Regulated APN-AMPK Signal Pathway of Steroid-induced Avascular Necrosis of Femoral Head Rabbits

作　　者：张翔[1] 吴泱 董晓俊[3] 张鸿振[1] 覃剑[3] 李冬[3] 朱一洲 戴子一 ZHANG Xiang;WU Yang;DONG Xiao-jun;ZHANG Hong-zhen;QIN Jian;LI Dong;ZHU Yi-zhou;DAI Zi-yi(Department of TCM Traumatology,Wenzhou Hospital of Integrative Medicine,Zhejiang University of Chinese Medicine,Zhejiang (325000);Department of TCM Internal Medicine,Wenzhou Hospital of Integrative Medicine,Zhejiang University of Chinese Medicine,Zhejiang (325000);Department of TCM Traumatology,Wuhan Hospital of TCM,Wuhan (430014))

机构地区：[1]浙江中医药大学附属温州市中西医结合医院中医骨伤科,浙江325000 [2]浙江中医药大学附属温州市中西医结合医院中医内科,浙江325000 [3]湖北省武汉市中医医院骨伤科,武汉430014

出　　处：《中国中西医结合杂志》2019年第10期1234-1239,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：湖北省中医药中西医结合科研项目(No.2013Z-Y12)

摘　　要：目的通过干预脂联素(APN)-单磷酸腺苷活化蛋白激酶(AMPK)信号转导通路,观察股骨头局部血管重建,探讨采用补肾活血方治疗激素性股骨头坏死(SANFH)的作用机制。方法 27只健康成年白兔,随机分为正常组、造模组和实验组,每组9只,应用大肠杆菌脂多糖联合醋酸泼尼松龙诱导SANFH模型。实验组每天采用补肾活血方(5 mL/kg)灌胃,其他两组每天采用生理盐水(5 mL/kg)灌胃,疗程均为8周。实验过程中记录动物一般情况,造模前后、用药8周末分别对所有兔耳缘静脉取血2 mL,采用酶联免疫吸附测定法(ELISA)检测APN水平;检测实验组用药前后肿瘤坏死因子-α(TNF-α)、骨保护素(OPG)水平和股骨头B淋巴细胞瘤-2基因(Bcl-2)、半胱氨酸蛋白酶-3(Caspase-3)表达情况,并在用药8周末进行X线检测。结果造模组造模后APN浓度水平较造模前及正常组均下降(P<0.05);实验组用药8周末APN水平较造模组升高(P<0.05);TNF-α免疫组化水平明显下降,OPG免疫组化水平升高,同时股骨头中Bcl-2表达较用药前明显升高,Caspase-3表达水平下降(P<0.05)。结论补肾活血方通过调节APN-AMPK信号传导通路中APN、TNF-α/OPG、Bcl-2/Caspase-3表达,对兔SANFH发挥有效干预。Objective To study the mechanism of tonifying Shen and activating blood circulation method for treating steroid-induced avascular necrosis of femoral head(SANFH) by interfering with adiponectin(APN)-adenosine monophosphate-activated protein kinase(AMPK) signal pathway and observing the local vascular reconstruction of femoral head. Methods Totally 27 healthy adult white rabbits were randomly divided into a normal group, a model group, and an experimental group, 9 rabbits in each group. SANFH model was induced by lipopolysacchatide(LPS) combined prednisolone acetate. Rabbits in the experimental group were administered with BSHXR(5 mL/kg), while rabbits in the other two groups were administered with normal saline(5 mL/kg). The treatment course for all was 8 weeks. During the experiment, the general conditions of the rabbits were recorded. Before and after modeling/at the end of the 8 th week of the experiment, 2 mL blood was collected from the ear vein of all rabbits. APN was detected by ELISA. Expressions of B-cell lymphoma-2(Bcl-2), tumor necrosis factor(TNF-α), Caspase 3, and osteoprotegerin(OPG) were detected in the experimental group by immunohistochemical assay before and after treatment. X-ray examination was performed at the end of the 8 th week of treatment. Results The APN concentration after modeling decreased in the model group, as compared with before modeling and the normal group(P<0.05). The APN concentration after 8 weeks of administration increased more in the experimental group than in the model group(P<0.05). The TNF-α expression significantly decreased and the OPG expression significantly increased. Meanwhile, the expression of Bcl-2 significantly increased and the expression of Caspase-3 obviously decreased(P<0.05). Conclusion BXHXR effectively intervened SANFH in rabbits by regulating the expressions of APN, TNF-α/OPG, Bcl-2/Caspase-3 in APN-AMPK signal pathway.

关 键 词：激素性股骨头坏死 APN-AMPK信号通路 动物模型 补肾活血法 

分 类 号：R285.5[医药卫生—中药学]