饲粮中添加甘露寡糖对肉仔鸡生长性能及组织天然免疫相关基因表达的影响  

Effects of Dietary Supplementation of Mannan-Oligosaccharides on Growth Performance and Innate Immunity-Associated Gene Expressions in Tissues of Broiler Chickens

作　　者：熊阿玲 包龙飞 许兰娇[1] 万根[1] 龙光莲 李君惠 黎观红[1] Xiong Ailing;Bao Longfei;Xu Lanjiao;Wan Gen;Long Guanglian;Li Junhui;Li Guanhong(Jiangxi Province Key Laboratory of Animal Nutrition;Engineering Research Center for Feed Development of Jiangxi Province,Jiangxi Agricultural University,Nanchang 330045)

机构地区：[1]江西农业大学江西省动物营养重点实验室江西省营养饲料开发工程研究中心

出　　处：《中国粮油学报》2019年第9期80-87,共8页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31360555,31560641);江西省自然科学基金(20161ACB20008)

摘　　要：旨在探讨饲粮中添加不同浓度甘露寡糖(MOS)对肉仔鸡生长性能及肝脏、脾脏、回肠和盲肠中Toll样受体(TLR)和抗菌肽等天然免疫基因表达的影响。选用176只1日龄雄性科宝-500白羽肉仔鸡,随机分成4个处理组,每组4个重复,每个重复11只鸡,四个处理组分别饲喂在基础饲粮中添加0.0、0.3、0.6和0.9 g/kg甘露寡糖的饲粮,试验时间为42 d。结果表明:与对照组相比,饲粮中添加0.3~0.9 g/kg MOS显著提高肉仔鸡1~42日龄的平均日采食量和平均日增重(P<0.05或P<0.01);饲粮添加MOS显著提高肉仔鸡21日龄法氏囊指数和42日龄胸腺指数(P<0.05)。与对照组相比,饲粮中添加MOS可不同程度地提高21和42日龄肉鸡回肠、盲肠、肝脏和脾脏TLR2、TLR4、β-防御素9(AvBD9)和cathelicidin-B1(Cath-B1)mRNA表达(P<0.05或P<0.01)。饲粮中添加适宜水平的MOS可不同程度地提高肉仔鸡生长性能,并可能通过提高肉仔鸡组织TLRs表达并由TLRs介导上调β-防御素和cathelicidins等抗菌肽表达而提高肉仔鸡天然免疫防御功能。This study aimed to investigate the effects of dietary supplementation of mannan-oligosaccharides (MOS) on growth performance, Toll-like receptors (TLR) and host defense peptides (HDP) gene expressions in liver, spleen, jejunum and caecum of broilers. A total of 176 one-day-old male Cobb-500 broilers were randomly allotted to one of 4 dietary treatments with 4 replicate pens of 11 broilers each for 42 days. The four dietary treatments were basal diet supplemented with 0.0 (control), 0.3, 0.6 and 0.9 g/kg, respectively. The results showed that, as compared with the control treatment, dietary supplementation of MOS at the concentrations ranging from 0.3 to 0.9 g/kg significantly increased average daily feed intake and average daily weight gain of broilers from 1 to 42 days of age (P<0.05 or P<0.01). When compared the control treatment, dietary supplementation of MOS significantly enhanced bursa of Fabricius index and thymus index of broilers on d 21 and 42 (P<0.05), respectively. Dietary supplementation of MOS increased mRNA expressions of TLR2, TLR4, avian β-defensin 9 (AvBD9) and cathelicidin-B1 (Cath-B1) in ileum, caecum liver and spleen of broilers on d 21 and 42 with different potential (P<0.05 or P<0.01). These results indicate dietary supplementation with MOS could improve growth performance, upregulate innate immunity-associated genes (TLR2, TLR4, AvBD9 and Cath-B1) expression in broilers, implying that MOS improves innate immune defense probably through TLRs-mediated upregulation of host defense peptides such as β-defensins and cathelicidins expression.

关 键 词：甘露寡糖 肉仔鸡 TOLL样受体 抗菌肽 

分 类 号：S831.1[农业科学—畜牧学]