Application of virus-induced gene silencing for identification of FHB resistant genes  

作　　者：FAN Yan-hui HOU Bing-qian SU Pei-sen WU Hong-yan WANG Gui-ping KONG Ling-rang MA Xin WANG Hong-wei 

机构地区：[1]State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy,Shandong Agricultural University,Tai'an 271018,P.R.China [2]Shandong Agricultural University Fertilizer Science Technology Co.,Ltd.,Feicheng 271600,P.R. China

出　　处：《Journal of Integrative Agriculture》2019年第10期2183-2192,共10页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (3315203911 and 31471488);the National Key Research and Development Program of China (2016YFD0100602);the Transgenic Special Item, China (2016ZX08002003-002 and 2016ZX08009-003);the Bohai Granary Science and Technology Demonstration Project of Shandong Province, China (2017BHLC020)

摘　　要：Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)- based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.Virus-induced gene silencing(VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus(BSMV)-based VIGS system to evaluate Fusarium head blight(FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, Ta AOC, Ta AOS, and Ta OPR3 involved in jasmonic acid(JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing Ta AOS in wheat by stable transgenic plants.

关 键 词：TRITICEAE FUSARIUM HEAD blight(FHB) BSMV-VIGS RNAI 

分 类 号：S[农业科学]