高效液相色谱法测定便秘糖衣丸中3种有效成分的含量  

Content determination of 3 active ingredients in Surulac-S by high performance liquid chromatography

作　　者：徐文峰[1] 金鹏飞[1] 徐硕[1] 姜文清[1] 吴学军[1] 邝咏梅[1] 徐巧玲[2] XU Wen-feng;JIN Peng-fei;XU Shuo;JIANG Wen-qing;WU Xue-jun;KUANG Yong-mei;XU Qiaoling(Department of Pharmacy,National Center of Gerontology,Beijing Hospital,Key Laboratory of Assessment of Clinical Drugs Risk and Individual Application,Bejing 100730;Department of Pharmacy,The 305th Hospital of the PLA of China,Bejing 100017)

机构地区：[1]北京医院药学部国家老年医学中心,药物临床风险与个体化应用评价北京市重点实验室,北京100730 [2]解放军第305医院药局,北京100017

出　　处：《中南药学》2019年第9期1525-1528,共4页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81803715);北京医院科技新星项目(No.BJ-2016-039)

摘　　要：目的建立高效液相色谱法测定便秘糖衣丸中比沙可啶、番泻苷A和番泻苷B的含量。方法采用Alltima C18色谱柱(150 mm×4.6 mm,5μm),乙腈-30 mmol·L^-1磷酸二氢铵溶液为流动相,流速为1.0m L·min^-1,比沙可啶的检测波长为265 nm,番泻苷A和番泻苷B的检测波长为340 nm。结果比沙可啶、番泻苷A与番泻苷B的线性范围分别为26.23~419.68μg·mL^-1、9.72~194.44μg·m L^-1、9.54~190.72μg·m L^-1,相关系数分别为0.9998、0.9997、0.9993,上述3种化学成分平均加样回收率分别为98.6%、97.5%、97.2%,RSD均小于3%。结论该方法可用于测定便秘糖衣丸中比沙可啶、番泻苷A和番泻苷B的含量。Objective To establish a high performance liquid chromatography(HPLC) method to determine the content of bisacodyl, sennoside A and sennoside B in Surulac-S. Methods An Alltima C18 column(150 mm×4.6 mm, 5 μm) was used for the separation with mobile phase of acetonitrile-30 mmol·L^-1 ammonium dihydrogen phosphate solution at 1.0 mL·min^-1. The detection wavelength was 265 nm for bisacodyl while 340 nm for both sennoside A and sennoside B. Results The linear ranges of bisacodyl, sennoside A and sennoside B were 26.23-419.68 μg·mL^-1, 9.72^-194.44 μg·mL^-1, and 9.54^-190.72 μg·mL^-1, with correlation coefficients of 0.9998, 0.9997 and 0.9993, respectively. The average recoveries of the 3 components were 98.6%, 97.5% and 97.2% with RSDs all below 3%. Conclusion The method is suitable for the determination of bisacodyl, sennoside A and sennoside B in Surulac-S.

关 键 词：便秘糖衣丸 比沙可啶 番泻苷A 番泻苷B 含量测定 

分 类 号：R917[医药卫生—药物分析学] R284[医药卫生—药学]