白花杜鹃水提液对多房棘球蚴的体外杀灭和体内感染疗效的研究  

Effect of Rhododendron mucronatum decoction against protoscolex in vitro and alveolar hydatids in vivo

作　　者：高艳 郝宝成 宋向东 续文君 李立[2] 闫鸿斌[2] 吴金措姆[3] 拉巴次旦[3] 田波 贾万忠[2] GAO Yan;HAO Bao-cheng;SONG Xiang-dong;XU Wen-jun;LI Li;YAN Hong-bin;Wujin Cuomu;Laba Cidan;TIAN Bo;JIA Wan-zhong(Key Laboratory of New A nimal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development ,Ministry of Agricultural and Rural Affairs/Lanzhou Institute of Husbandry and Pharmaceutical Sciences , Chinese Academy of Agriculture Sciences , Lanzhou 730050, China;State Key Laboraiory on Veterinary Etiological Biology/Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences , Lanzhou 730046, China;Tibet Livestock Research Institute , Tibet Academy of Agriculture and Animal Husbandry Sciences , Lhasa 850009, China;China Agricultural Vet. Bio. Science and Technology Co.,Ltd., Lanzhou 730046, China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [3]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [4]中农威特生物科技股份有限公司,甘肃兰州730046

出　　处：《中国兽医科学》2019年第10期1331-1339,共9页Chinese Veterinary Science

基　　金：中央级公益性科研院所基本科研业务费专项(1610312017001;1610312016012)

摘　　要：为研究白花杜鹃水提液对多房棘球蚴--原头蚴的体外杀灭作用,以及对多房棘球蚴感染小鼠的治疗作用,通过在体外原头蚴培养体系中分别添加白花杜鹃水提液生药质量浓度分别为10、20和40 mg/mL,以阿苯达唑伊维菌素培养基(阿苯达唑的质量浓度为20 mg/mL、伊维菌素的质量浓度为0.83 mg/mL)、阿苯达唑伊维菌素溶剂培养基和常规培养基分别作为对照组进行原头蚴的培养,分别经第0、12小时和第1、2、3、4、5、6、7天观察记录各药物对原头蚴的杀灭效果,并于第7天通过台盼蓝染色比较各组死亡率;将50只感染多房棘球蚴1个月的雌性BALB/c小鼠随机分为5组,每组10只,依次为白花杜鹃水提液低剂量(10 mg/mL,RL组)、中剂量(20 mg/mL,RM组)、高剂量(40 mg/mL,RH组)治疗组,阿苯达唑伊维菌素治疗组(C组),感染不治疗组(IT组),另包括10只健康小鼠作为空白对照组(B组),各组小鼠连续灌胃给药后第7天,第30天后剖检,对小鼠脏器及棘球蚴组织固定并做石蜡切片进行病理学观察。结果显示,白花杜鹃水提液低、中、高剂量组,阿苯达唑伊维菌素溶液组,溶剂对照组以及空白对照组原头蚴培养后第7天后,原头蚴的死亡率分别为54.00%、56.53%、89.27%、57.60%、19.27%和15.80%,白花杜鹃高剂量组与对照组相比差异极显著(P<0.01)。多房棘球蚴感染小鼠腹部明显肿胀,剖检可见腹腔内有大量大小不等的包囊。石蜡切片结果表明,RH组小鼠脏器病变程度最低,且RH组和C组小鼠体内少量包囊发生钙化。研究表明,白花杜鹃水提液对体外原头蚴具有杀灭作用,且40 mg/mL白花杜鹃水提液对小鼠感染多房棘球蚴有一定的治疗作用。To study the killing effection of Rhododendron mucronatum decoction against cultured protoscolex in vitro and the treatment effects in mouse infected with alveolar hydatids. The test was carried out by adding R.mucronatum with 10,20 and 40 mg/mL,respectively,and albendazole ivermectin powder solution(wherein the concentration of albendazole and ivermectin was 20 mg/mL and 0.83 mg/mL,respectively) to the cultured system of alveolar hydatids,and the killing effect was observed and recorded at 0 h,12 h and on day 1,2,3,4,5,6 and 7,respectively.Fifty BALB/c mice,kept as the model of alveolar hydatid infection,were divided into five groups,with ten mice in each group,followed by 10 mg/mL(low,group RL),20 mg/mL(medium,group RM),40 mg/mL(high,group RH) doses R.mucronatum decoction treatment groups,albendazole ivermectin treatment group(group C),and infection control group without treatment(group IT),one blank control group(B) included with ten normal mice.The mice of each group were continuously administered by gavage for 7 days.On day 30,the mice were dissected and the pathological tissue was observed.The protoscolex cultured studied showed that after treated for 7 d,the mortality rate of low-,medium-,and high-dose R.mucronatum groups,albendazole and ivermectin group,solvent control group and blank control group were 54.00%,56.53%,89.27%,57.60%,19.27% and 15.80%,respectively.The high dose group of R.mucronatum decoction was significantly different from the blank group(P<0.01).The abdomen of the mice infected with alveolar hydatid was obviously swollen,and a large number of cysts with different sizes were found in the abdominal cavity.The results of paraffin section showed that the RH group had the lowest degree of pathological changes in the organs of BALB/c mice in different experimental groups,and the small amount of cystic calcification was observed in RH and C groups.These results showed that R.mucronatum decoction had a killing effect in vitro,and that 40 mg/mL decoction had a certain inhibitory effect on mice inf

关 键 词：白花杜鹃水提液 多房棘球蚴 小鼠 原头蚴 杀灭率 钙化 

分 类 号：S853.7[农业科学—临床兽医学]