扬州市儿童腹泻样本中3株salivirus 病毒基因组特征分析  

Genetic characteristics of three strains of salivirus in infants with diarrhea in Yangzhou city of China

作　　者：张倩[1] 苏璇 赵康辰[3] 朱小娟[3] 王艳 徐勤 郑东宇[3] 唐震[3] 王祥喜 庞正 崔仑标[3] 朱凤才[3] ZHANG Qian;SU Xuan;ZHAO Kang-chen;ZHU Xiao-juan;WANG Yan;XU Qin;ZHENG Dong-yu;TANG Zhen;WANG Xiang-xi;PANG Zheng;CUI Lun-biao;ZHU Feng-cai(Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学,天津300071 [2]扬州市疾病预防控制中心,江苏扬州225001 [3]江苏省疾病预防控制中心国家卫生健康委员会肠道病原微生物重点实验室,江苏南京210009

出　　处：《中国病毒病杂志》2019年第5期342-346,共5页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金(81871666);江苏省自然科学基金(BK20161583);江苏省第五期“333高层次人才培养工程”科研资助项目(BRA2017552)

摘　　要：目的分析3株salivirus病毒基因组特征,为了解我国salivirus病毒分子进化特征提供数据。方法对2018年扬州市儿童腹泻病例中3份salivirus病毒检测阳性的样本核酸进行高通量测序,获得该病毒的全基因组,应用MEGA 7.0软件构建系统进化树,通过MegAlign软件对核苷酸及编码区氨基酸序列进行同源性分析。结果 3株salivirus病毒均属于salivirus A1亚型,病毒之间基因组核苷酸及编码区氨基酸同源性均为99.4%~99.7%。与匈牙利毒株Hu/Baja-5332/2013/HUN/Salivirus A的同源性最高,核苷酸和编码区氨基酸同源性分别为96.5%~96.7%及98.0%~98.1%,处于同一进化分支。3株salivirus与国内salivirus A1亚型毒株的核苷酸和编码区氨基酸同源性分别为94.2%~95.2%及95.2%~97.1%;与国外salivirus A1亚型的核苷酸和编码区氨基酸的同源性分别为89.7%~96.7%及95.0%~98.5%,与国外salivirus A2亚型的核苷酸和编码区氨基酸的同源性分别为81.8%~81.9%及88.3%~88.4%。结论 3株salivirus病毒遗传进化特征不同于我国已报道的salivirus病毒,丰富了我国salivirus病毒的基因数据库。Objective To study the genomic characteristics of three strains of salivirus in Yangzhou city of China. Methods The genome of the three strains of salivirus isolated from diarrhea cases in 2018were obtained by high-throughput sequencing.Phylogenetic tree was constructed by MEGA 7.0software.Homology analysis of nucleotide and amino acids were conducted by MegAlign software. Results The three strains of salivirus belong to the salivirus A1subtype;the homology of nucleotides and the coding region amino acids were both 99.4%-99.7%.The sequences showed highest homology with that of Hungary strain Hu/baja- 5332/2013/HUN/Salivirus A.The homology of nucleotides and coding region amino acids were 96.5%-96.7% and 98.0%-98.1%,respectively.Phylogenitic anylsis showed they were in the same evolutionary branch.The nucleotide and coding amino acid homology of three strains of salivirus compared with China reference strains of salivirus A1subtype were 94.2%-95.2% and 95.2%-97.1%,respectively;89.7%-96.7% and 95.0%- 98.5%,respectively when compared with foreign salivirus A1subtype;81.8%-81.9% and 88.3%-88.4%,respectively when compared with salivirus A2. Conclusions The genetic and evolutionary characteristics of three strains of salivirus isolated in Yangzhou city of China are different from those reported previously in China.

关 键 词：salivirus 病毒 基因组 分子进行特征 

分 类 号：R725.7[医药卫生—儿科]