2009—2015年广东地区少数民族献血者丙型肝炎病毒基因型分布的研究  

Distribution of hepatitis C virus genotypes in minorities among volunteer blood donors in Guangdong province of China

作　　者：许茹[1,2] 杜荣松 王敏[1,2] 黄杰庭 廖峭[1,2] 单振刚 戎霞[1,2] 付涌水 XU Ru;DU Rong-song;WANG Min;Huang Jie-ting;LIAO Qiao;SHAN Zhen-gang;RONG Xia;FU Yong-shui(Guangzhou Blood Center,Guangzhou,Guangdong 510095,China)

机构地区：[1]广州血液中心,广东广州510095 [2]广州市医学重点实验室(血液安全重点实验室),广东广州510095 [3]南方医科大学输血医学系,广东广州510515

出　　处：《中国病毒病杂志》2019年第5期351-356,共6页Chinese Journal of Viral Diseases

基　　金：广东省医学科学技术研究基金项目(A2018499);广州市医学重点学科建设项目([2016]27号)

摘　　要：目的了解广东地区少数民族献血者的丙型肝炎病毒(HCV)基因型分布以及不同基因型之间的进化距离。方法本研究收集2009-2015年广东地区少数民族献血者的HCV RNA阳性标本共44份,对其进行HCV E1基因的巢式PCR扩增并测序获得序列。通过MEGA 7.0对上述标本进行基因分型,采用p-distance对广东地区汉族和少数民族感染的HCV的进化距离进行计算,并分析广东地区少数民族和汉族的基因分布差异。结果共获得少数民族献血者40条HCV E1基因序列,经分析其基因型主要为1b(13个)和6a(12个),其他依次为3a(5个)、3b(4个)、1a(3个)、2a(2个)和4a(1个)。相对于1b(χ^2=13.2,P<0.01)、3b(χ^2=4.37,P<0.05)、3a(χ^2=6.0,P<0.05)、2a(χ^2=10.98,P<0.01)和6a(χ^2=11.15,P<0.01),亚型4a在少数民族中的分布远远高于其在汉族中的分布。相对于1b(χ^2=19.07,P<0.01)、3b(χ^2=4.51,P<0.05)、3a(χ^2=6.86,P<0.01)、2a(χ^2=11.42,P<0.01)和6a(χ^2=15.69,P<0.01),亚型1a在少数民族中的分布远远高于其在汉族人群中的分布。少数民族感染的HCV进化距离远远大于汉族感染的HCV进化距离(t=-10.01,P<0.01)。结论广东地区少数民族感染的HCV基因型分布和汉族的基因型分布不同,并且,少数民族感染的HCV之间的进化距离大于汉族感染的HCV进化距离。Objective To study the distribution of hepatitis C virus(HCV)genotypes in minorities in Guangdong province of China. Methods Forty-four HCV RNA positive volunteer blood donor samples were collected from 2009to 2015at the Guangzhou Blood Center.HCV E1sequences were obtained by nested PCR.HCV genotypes were determined by MEGA 7.0and p-distance was calculated.The difference between Minorities and Han ethnic were analysis by SPSS 16.0. Results The predominant genotype in minorities in Guangdong province of China were 1band 6a,followed by 3a,3b,1a,2aand 4a.The prevalence of subtype 4a was higher in minorities than that in Han ethnic when compared with 1b(χ^2=13.2,P<0.01),3b(χ^2= 4.37,P<0.05),3a(χ^2=6.0,P<0.05),2a(χ^2=10.98,P<0.01)and 6a(χ^2=11.15,P<0.01).In addition,the prevalence of subtype 1awas also higher in minorities than that in Han ethnic when compared with 1b(χ^2=19.07,P<0.01),3b(χ^2=4.51,P<0.05),3a(χ^2=6.86,P<0.01),2a(χ^2=11.42,P<0.01)and 6a(χ^2=15.69,P<0.01).The p-distance of HCV in minorities was greater than that in Han ethnic(t=-10.01,P<0.01). Conclusions There is a significant difference of HCV genotypes distributed between Han ethnic and minorities.Furthermore,the p-distance of HCV in minorities was greater than that in Han ethnic.

关 键 词：丙型肝炎病毒 进化树 基因型 进化距离 少数民族 

分 类 号：R512.63[医药卫生—内科学]