还原型谷胱甘肽对兔自体脂肪移植存活的影响  

Effect of glutathione on autologous fat graft survival of the rabbits

作　　者：吴亮亮[1] 马莉[1] 蒋思静 贾大平 柳芳 高堂俊 赵宇[1] Wu Liangliang;Ma Li;Jiang Sijing;Jia Daping;Liu Fang;Gao Tangjun;Zhao Yu(Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China)

机构地区：[1]安徽医科大学第一附属医院整形外科,合肥230022

出　　处：《中华整形外科杂志》2019年第9期928-933,共6页Chinese Journal of Plastic Surgery

基　　金：安徽省转化医学研究院科研基金(2017zhuyx05).

摘　　要：目的探讨在肿胀麻醉液中加入还原型谷胱甘肽对兔自体脂肪移植存活的影响.方法 14只雄性和雌性新西兰兔按随机数字表法平均分为实验组和对照组,每组7只.实验组:新西兰兔供区注射3 ml加入还原型谷胱甘肽的肿胀麻醉液;对照组:新西兰兔供区注射3 ml常规肿胀麻醉液.提取新西兰兔颈肩胛区脂肪组织,进行兔耳自体颗粒脂肪移植.并采用2,7-双乙酸二氯荧光素探针荧光染色提取的自体脂肪组织细胞,荧光酶标仪检测细胞活性氧含量.移植3个月后,将移植物取出进行观察和分析:(1)大体观察;(2)测量湿重;(3)测量体积;(4)HE染色,计数脂肪细胞数;(5)CD34免疫组织化学法染色,测量微血管密度.使用SPSS 24.0进行t检验.结果实验组提取的自体脂肪组织细胞活性氧含量小于对照组,差异有统计学意义(P<0.001).移植3个月后,移植物湿重结果实验组为(1133.21±87.97)mg,对照组为(718.79±79.27)mg.体积结果实验组为(1.00±0.04)ml,对照组为(0.59±0.03)ml.实验组脂肪细胞数为(746.6±15.7)个/10个高倍镜视野,对照组为(350.1±32.4)个/10个高倍镜视野.微血管密度实验组为(8.1±2.0)个/高倍镜视野,对照组为(6.7±2.4)个/高倍镜视野.实验组移植物的体积、湿重和脂肪细胞数均大于对照组,差异有统计学意义(P<0.05).实验组与对照组微血管密度差异无统计学意义(P>0.05).结论还原型谷胱甘肽在肿胀麻醉液中的应用优化了兔自体脂肪的获取过程,从而提高了移植后脂肪的存活率.Objective To investigate the effect of adding glutathion( GSH) to tumescent solution on autologous fat graft survival. Methods 14 male and female New Zealand rabbits were divided into experimental group and control group randomly, 7 in each group. Experimental group:The donor areas of the rabbits were injected with 3 ml of tumescent solution with GSH. Control group:The donor areas of the rabbits were injected with 3 ml regular tumescent solution. DCFH-DA probe was used for fluorescent staining of harvested fat cells. Then stained fat cells were measured for the intracellular reactive oxygen species ( ROS ) content by fluorescence microplate. The grafts were harvested at 3 months after transplantation and assessed by general observation, volume measurement, wet weight measurement, HE staining for the number of fat cells, and CD34 immunohistochemical staining for the measurement of micro-vascular density. T test was performed by using SPSS 24. 0. Results The intracellular ROS content of harvested fat cells in experimental group was lower than that in control group, and the difference was statistically significant ( P<0. 05 ). At 3 months after transplantation, the wet weight of fat grafts in experimental group was (1133. 21±87. 97) mg and that in control group was (718. 79±79. 27) mg. The volume of fat grafts in experimental group was ( 1. 00 ± 0. 04) ml and that in control group was ( 0. 59 ± 0. 03) ml. The number of fat cells in experimental group was (746. 6±15. 7)/10 high magnification vision and that in control group was ( 350. 1 ± 32. 4 )/10 high magnification vision. The density in group experimental was (8. 1±2. 0)/high magnification vision and that in control group was (6. 7±2. 4)/high magnification vision. The grafts'volume, wet weight, and the number of fat cells in experimental group were higher than those in control group, and the differences were statistically significant (P<0. 05). The difference of micro-vascular density between experimental group and control group had no statistical signifi

关 键 词：还原型谷胱甘肽 自体脂肪移植 肿胀麻醉液 氧化应激 

分 类 号：R622[医药卫生—整形外科]