Photosynthetic traits and antioxidative defense responses of Pinus yunnanensis after joint attack by bark beetles Tomicus yunnanensis and T.minor  

作　　者：Juan Liu Hang Chen Jianmin Wang Xiaoming Chen Zixiang Yang Junsheng Liang 

机构地区：[1]The Research Institute of Resource Insects,Chinese Academy of Forestry,Kunming,People’s Republic of China [2]Key Laboratory of Breeding and Utilization of Resource Insects of State Forestry Administration,Kunming 650224,Yunnan,People’s Republic of China [3]Hunan Academy of Forestry,Changsha 410004,Hunan,People’s Republic of China

出　　处：《Journal of Forestry Research》2019年第6期2031-2038,共8页林业研究（英文版）

基　　金：supported by the Fundamental Scientific Research Fund for Central Non-profit Research Institute(Grant No.CAFYBB2016MA006);the Public Welfare Special Fund Project of Forestry Industry of State Forestry Administration of China(Grant No.200904052)

摘　　要：Bark beetles Tomicus yunnanensis and T.minor are two important pests of Pinus yunnanensis and can cause massive death of pine trees.In this study,we examined several traits related to photosynthesis in P.yunnanensis and their relationship with antibiotic defense responses after joint attack by the two bark beetles at the shoot and the trunk stages.When shoots were attacked by the beetles,the abundance of chlorophylls,carotenoids,and the rates of net photosynthesis(Pn)and transpiration(E)decreased in needles,while the levels of superoxide dismutase and malondialdehyde remained unchanged in both needles and phloem.The activity of peroxidases also remained unchanged in needles,but increased in phloem.The activity of catalases increased in both needles and phloem.When trunks were attacked by the bark beetles,chlorophyll abundance,Pn,E,and antioxidative enzyme activities all declined,and the declines were more pronounced than in the attacked shoots.A decrease in protein concentrations was also observed in needles and phloem from the attacked pines.Attack on shoots by the bark beetles suppressed host defense and provided a favorable environment for larval growth and development,resulting in long-term decline of pine growth potential.The results suggest that attacks on trunks by beetles caused more severe damage to host trees than attacks on shoots.Bark beetles Tomicus yunnanensis and T. minor are two important pests of Pinus yunnanensis and can cause massive death of pine trees. In this study, we examined several traits related to photosynthesis in P.yunnanensis and their relationship with antibiotic defense responses after joint attack by the two bark beetles at the shoot and the trunk stages. When shoots were attacked by the beetles, the abundance of chlorophylls, carotenoids, and the rates of net photosynthesis(Pn) and transpiration(E) decreased in needles, while the levels of superoxide dismutase and malondialdehyde remained unchanged in both needles and phloem. The activity of peroxidases also remained unchanged in needles, but increased in phloem.The activity of catalases increased in both needles and phloem. When trunks were attacked by the bark beetles,chlorophyll abundance, Pn, E, and antioxidative enzyme activities all declined, and the declines were more pronounced than in the attacked shoots. A decrease in protein concentrations was also observed in needles and phloem from the attacked pines. Attack on shoots by the bark beetles suppressed host defense and provided a favorable environment for larval growth and development, resulting in long-term decline of pine growth potential. The results suggest that attacks on trunks by beetles caused more severe damage to host trees than attacks on shoots.

关 键 词：Antioxidant enzymes BARK BEETLE Defense responses Photosynthesis PINUS yunnanensis Tomicus MINOR Tomicus yunnanensis 

分 类 号：S[农业科学]