山药根际土壤微生物16S rRNA多样性及影响因素  

Genetic 16S rRNA Diversity of Soil Microbes in Rhizosphere of Chinese Yam and Its Influencing Factors

作　　者：张红霞 张舒雅[1] 张玉涛 张天缘 ZHANG Hongxia;ZHANG Shuya;ZHANG Yutao;ZHANG Tianyuan(College of Chemistry and Chemical Engineering,Anshun University,Anshun,Guizhou 561000,China;Engineering Technology Center of Control and Remediation of Soil Contaminationof Provincial Science & Technology Bureau,Anshun University,Anshun,Guizhou 561000,China;Wuhan Benagen Technology Solutions Company Limited,Wuhan 430070,China)

机构地区：[1]安顺学院化学化工学院,贵州安顺561000 [2]安顺学院/贵州省科技厅土壤污染控制与修复工程技术中心,贵州安顺561000 [3]武汉贝纳科技服务有限公司,湖北武汉430070

出　　处：《土壤学报》2019年第5期1235-1246,共12页Acta Pedologica Sinica

基　　金：国家自然科学基金项目（41403114）;贵州省科技厅三方联合基金项目（黔科合J字LKA[2013]06，黔科合LH[2016]7278）资助~~

摘　　要：通过采集山药连作2年的植株根际土壤及根茎,采用高通量测序技术研究根际土壤细菌多样性,分析7个样本中菌群的组成、丰度、α多样性、β多样性、菌群差异性。采用典型相关分析(Canonical Correlation Analysis,CCA)分析优势菌群与土壤化学因子、根茎糖类物质的关系,探讨根际土壤中细菌群落与土壤化学指标、根茎糖类物质的相关性。结果表明:山药根际土壤细菌优势群落为变形菌、绿弯菌、酸杆菌、放线菌;土壤pH是影响细菌群落结构及分布的重要因子;酸杆菌和硝化螺旋菌与土壤中蔗糖酶、脲酶活性及总有机碳、碱解氮呈正相关,奇古菌和绿弯菌与土壤总有机碳、碱解氮呈正相关,土壤中碳、氮养分又与山药根茎中可溶性糖、粗多糖及淀粉呈正相关关系,说明这些微生态环境因子可能对山药根茎糖类物质积累有促进作用。【Objective】Chinese yam (Dioscorea opposita) is a parannial herbal liane popular as vegetable in this country. Its tuber is consumed as both food and medicinal herb for its functions of antioxidation, anti-aging, immunity regulation, blood sugar reduction, etc. Soil ecoenvironmental factors and soil nutrient status are major factors affecting growth and quality of the plant. Soil microbes, in particular, participate in recycling of carbon, nitrogen and some other nutrient elements in the soil, flowing of energy, formation of soil humus, shaping and amelioration of soil structure. This study aims to explore relationships of the soil bacterial community in the rhizosphere of yam with soil nutrients and contents of saccharides in the tuber, and to elucidate major soil ecological factors affecting nutrient accumulation in yam tuber, in an attempt to provide certain reference for development of yam-specfic bio-manure and assessment of soil environment for cultivation of the plant.【Method】Seven samples of rhizosphere soil and rhizomes of 2-year old Chinese yam plants were collected for analysis of composition, abundance, Alpha diversity, Beta diversity and bacteria diversity of soil microbes with the aid of the high-throughput sequencing technology, and for analysis of relationships dominant soil bacterial groups and soil microbial community in rhizosphere with soil chemical factors and saccharides in root with the canonical correlation analysis method.【Result】Results show that Shannon and Chaol indices were significantly and negatively related to soil phosphatese activity;proteobacteria, chloroflexi, acidobacteria and actinobacteria were the dominant bacterial groups in the rhizosphere of Chinese yam;soil pH was an important factor affecting structure and distribution of the soil bacterial community;acidobacteria and nitrifying spirulina was related to sucrase and urease activities in soil;archaea and chloroflexi was positively related to soil organic carbon and nitrogen;soil carbon, nitrogen nutrient was p

关 键 词：山药 根际土壤 细菌群落 多样性 相关性 

分 类 号：S154.3[农业科学—土壤学]