5'-单磷酸腺苷活化蛋白激酶活性降低对青春期小鼠乳腺发育的影响  

Effect of decreased activity of 5'-adenosine monophosphate activated protein kinase on breast development in adolescent mice

作　　者：王健[1] 吕健东[1] 安雪青 Wang Jian;Lyu Jiandong;An Xueqing(Department of General Surgery,the Fifth Tianjin Central Hospital,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院普外科,300450

出　　处：《中华实验外科杂志》2019年第10期1788-1790,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81202275).

摘　　要：目的观察小鼠乳腺上皮单磷酸腺苷活化蛋白激酶(AMPK)活性降低对青春期小鼠导管上皮细胞增殖及终端末梢(TEB)发育的影响.方法成功构建AMPK DN转基因(DN)小鼠,取野生型(WT)和DN组各10只小鼠右侧腹部第四对的乳腺组织进行全组织染色和增殖细胞核抗原(PCNA)免疫组织化学染色,分析WT和DN小鼠乳腺组织的导管覆盖面积、导管初始长度、每毫米导管分支数、导管侵袭长度百分数、TEB平均数量、TEB平均最大面积.并计算PCNA阳性细胞数与腺体上皮细胞总数目的比值.结果第5周时,DN小鼠与WT小鼠每毫米导管分支数[(1.39±0.18) mm比(1.21 ±0.11) mm]、TEB平均数量[(13.99 ±3.14)个比(10.65±2.09)个]、TEB平均最大面积[(0.104±0.014) mm2比(0.082±0.013)mm2]比较,差异有统计学意义(t=2.698、2.800、3.642,P<0.05);第6周时,DN小鼠与WT小鼠每毫米导管分支数[(1.73±0.31) mm比(1.38±0.34) mm]、TEB平均数量[(15.86±3.26)个比(12.47±2.34)个]、TEB平均最大面积[(0.118 ±0.017) mm2比(0.087±0.014) mm2],差异有统计学意义(￡=2.406、2.671、4.451,P<0.05);第5周DN小鼠乳腺上皮细胞PCNA阳性细胞占比(86.47 ±4.67)显著高于WT组的(65.32±3.87)%,第6周DN小鼠乳腺上皮细胞PCNA阳性细胞占比(85.37±4.35)显著高于WT组的(63.47±3.45)%,差异均有统计学意义(t=4.614、12.474,P<0.05).结论小鼠乳腺上皮AMPK活性降低对青春期小鼠导管上皮细胞增殖及TEBs发育具有促进作用.Objective To investigate the effects of decreased 5’-activated protein kinase (AMPK) activity in mouse mammary epithelium on the proliferation of ductal epithelial cells and the development of teminal end bud (TEB) in adolescent mice. Methods AMPK DN transgenic mice were successfully constructed. The fourth pair of mammary gland tissues from the right abdomen of 10 WT and DN mice were stained by whole tissue staining and PCNA immunohistochemical staining. The duct coverage area, initial duct length, number of ductal branches per millimeter, percentage of ductal invasion length, terminal tip of mammary gland tissues of WT and DN mice were analyzed. TEB average quantity, average maximum area of TEB. The ratio of PCNA positive cells to the total number of glandular epithelial cells was calculated. Results At 5th week, the number of ductal branches per millimeter in DN mice and WT mice was (1.39±0.18) mm vs.(1.21±0.11) mm, the average number of TEB was (13.99±3.14) vs.(10.65±2.09) and the average maximum area of TEB was (0.104±0.014) mm2 vs.(0.082±0.013) mm2, respectively, with significant difference (t=2.698, 2.800, 3.642, P<0.05);at 6th week, the number of ductal branches per millimeter in DN mice and WT mice was (1.59±0.013) mm2, respectively. 31) vs.(1.41±0.34) mm, the average number of TEB (15.86±3.26) vs.(12.47±2.34), the average maximum area of TEB (0.118±0.017) mm2vs.(0.087±0.014) mm2, the difference was statistically significant (t=2.406, 2.671, 4.451, P<0.05). The percentage of PCNA positive cells in mammary epithelial cells of 5 w DN mice (86.47±4.67) was significantly higher than that of WT mice (65.32±3.87)%, and 6 w DN mice (65.32±3.87)%. The percentage of cells in WT group was significantly higher than that in WT group (85.37±4.35)(63.47±3.45)%. The difference was statistically significant (t=4.614, 12.474, P< 0.05). Conclusion The decrease of AMPK activity in mouse mammary epithelium can promote the proliferation of ductal epithelial cells and the development of TEBs in adolescent m

关 键 词：小鼠乳腺上皮细胞 青春期 单磷酸腺苷活化蛋白激酶 增殖 终端末梢 

分 类 号：R346[医药卫生—基础医学] R-332