高效液相色谱法同时测定安坤胶囊中栀子苷、芍药苷、特女贞苷、蟛蜞菊内酯和丹皮酚的含量  

Simultaneous determination of the content of geniposide, paeoniflorin, specnuezhenide, wedelolactone and paeonol in Ankun capsules by HPLC

作　　者：任莹 范姣姣[2] 张鑫 REN Ying;FAN Jiao-jiao;ZHANG Xin(Xi'an Institute for Food and Drug Control,Xi'an 710054;Department of Pharmacy,Xi'an Children's Hospital,Xi'an 710003)

机构地区：[1]西安市食品药品检验所,西安710054 [2]西安市儿童医院药剂科,西安710003

出　　处：《中南药学》2019年第10期1699-1702,共4页Central South Pharmacy

摘　　要：目的建立HPLC法同时测定安坤胶囊中栀子苷、芍药苷、特女贞苷、蟛蜞菊内酯和丹皮酚含量。方法采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长230 nm(0~30 min)、351 nm(30~40 min)、274 nm(40~50 min);流速1.0mL·min^-1;柱温30℃,进样量:10μL。结果栀子苷、芍药苷、特女贞苷、蟛蜞菊内酯和丹皮酚分别在81.83~4091.69 ng(r=0.9999)、79.71~3985.61 ng(r=0.9999)、15.58~778.91 ng(r=0.9999)、1.35~67.40 ng(r=0.9992)、16.54~827.20 ng(r=0.9998)与峰面积线性关系良好;平均回收率分别为98.5%、97.2%、97.9%、102.9%、97.6%,RSD值分别为0.80%、1.0%、1.6%、2.6%、1.9%。结论该方法简便、快速、重复性好,可用于安坤胶囊的质量控制。Objective To establish an HPLC method for the simultaneous determination of the content of geniposide, paeoniflorin, specnuezhenide, wedelolactone and paeonol in Ankun capsules. Methods Agilent TC-C18 column(4.6 mm×250 mm, 5 μm) was used with temperature at 30 ℃. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The detection wavelengths were 230 nm(0-30 min), 351 nm(30-40 min) and 274 nm(40-50 min) at 1.0 mL·min^-1, and the volume of injection was 10 μL. Results The linearities of geniposide, paeoniflorin, specnuezhenide, wedelolactone and paeonol were 81.83-4091.69 ng(r = 0.9999), 79.71-3985.61 ng(r = 0.9999), 15.58-778.91 ng(r = 0.9999), 1.35-67.40 ng(r = 0.9992), and 16.54-827.20 ng(r = 0.9998), respectively. The average recoveries were 98.5%, 97.2%, 97.9%, 102.9% and 97.6% with RSD 0.80%, 1.0%, 1.6%, 2.6% and 1.9%, respectively. Conclusion The method is rapid, simple, reproducible, and can be used for the quality control of Ankun capsules.

关 键 词：安坤胶囊 栀子苷 芍药苷 特女贞苷 蟛蜞菊内酯 丹皮酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]