石斛酚通过NF-κB/PRL-3通路抑制骨肉瘤细胞增殖、迁移和侵袭  

Gigantol inhibits the proliferation, migration and invasion of osteosarcoma cells by NF-κB/PRL-3 pathway

作　　者：范兆阳[1] 鲜文峰[1] 刘永喜 张超[2] FAN Zhaoyang;XIAN Wenfeng;LIU Yongxi;ZHANG Chao(Department of Orthopedics,the Second People's Hospital of Nanyang City,Nanyang 473000,Henan,China;Department of Orthopedics,Henan Provincial People's Hospital,Zhengzhou 450003,Henan,China)

机构地区：[1]南阳市第二人民医院骨科,河南南阳473000 [2]河南省人民医院骨科,河南郑州450003

出　　处：《中国肿瘤生物治疗杂志》2019年第10期1095-1100,共6页Chinese Journal of Cancer Biotherapy

基　　金：河南省科技发展计划资助项目（No.142102310453）~~

摘　　要：目的:探讨石斛酚对人成骨肉瘤U20S细胞增殖、迁移及侵袭的抑制作用及其分子机制。方法:用不同质量浓度(10、25、50、75、100、150μmol/L)的石斛酚处理U20S细胞24、48h后,用CCK-8法检测石斛酚对U20S细胞增殖能力的影响。Transwell实验检测25、50μmol/L的石斛酚对U20S细胞迁移、侵袭能力的影响,在脂多糖(LPS)诱导U20S细胞炎症反应的基础上,再以石斛酚处理,qPCR、WB法分别检测U20S细胞中NF-κB(p65)、TNF-α、IL-6、PRL-3mRNA和蛋白的表达水平。结果:不同质量浓度的石斛酚作用不同时间对肉瘤细胞U20S增殖均具有抑制作用(P<0.05或P<0.01);25、50μmol/L的石斛酚能够明显抑制骨肉瘤U20S细胞的迁移及侵袭能力(均P<0.01),同时能够抑制细胞中NF-κB、TNF-α、IL-6、PRL-3等蛋白的表达(P<0.05或P<0.01);LPS诱导后,U20S细胞中NF-κB、TNF-α、IL-6、PRL-3mRNA和蛋白表达水平均显著升高(均P<0.01),25、50μmol/L的石斛酚处理后均能够降低U20S细胞中NF-κB、TNF-α、IL-6、PRL-3mRNA和蛋白的表达水平(P<0.05或P<0.01)。结论:石斛酚对骨肉瘤U20S细胞增殖、迁移和侵袭具有抑制作用,其作用机制可能与调控NF-κB/PRL-3信号通路有关。Objective:To study the inhibitory effect of gigantol on proliferation,migration and invasion of human osteosarcoma U20S cells and to explore the mechanism.Methods:After being treated with different concentrations(10,25,50,75,100,150μmol/L)of gigantol for 24 and 48 h,the proliferation of U20S cells was detected by CCK-8 assay.Transwell assay was used to detect the effects of 25μmol/L and 50μmol/L gigantol on the migration and invasion abilities of U20S cells.The lipopolysaccharide(LPS)was used to induce inflammatory reaction in U20S cells before gigantol treatment;qPCR and WB were used to detect the mRNA and protein expressions of NF-κB(p65),TNF-α,IL-6 and PRL-3,respectively.Results:Different concentrations of gigantol could all inhibit the proliferation of sarcoma U20S cells at different time(P<0.05 or P<0.01).The 25μmol/L and 50μmol/L of gigantol could significantly inhibit the migration and invasion of osteosarcoma U20S cells(all P<0.01);at the same time,it could inhibit the protein expressions of NF-κB,TNF-α,IL-6 and PRL-3(P<0.05 or P<0.01).After LPS induction,the mRNA and protein expressions of NF-κB,TNF-α,IL-6 and PRL-3 in U20S cells were significantly increased(all P<0.01);however,the consequent treatment with gigantol(25 and 50μmol/L)reversed the effects of LPS on U20S cells obviously(P<0.05 or P<0.01).Conclusion:Gigantol can inhibit the proliferation,migration and invasion of osteosarcoma U20S cells,and its mechanism may be related to the regulation of NF-κB/PRL-3 signaling pathway.

关 键 词：石斛酚 骨肉瘤 U20S细胞 增殖 侵袭 迁移 NF-ΚB PRL-3 

分 类 号：R738.1[医药卫生—肿瘤] R730.52[医药卫生—临床医学]