肥胖基因多态性与代谢综合征痰证病位、病性兼杂规律及理化指标相关性研究  

Study on Correlation of FTO Gene Polymorphism and Characteristic Regularity of Location, Nature, Physicochemical Indexes of Metabolic Syndrome with Phlegm Syndrome

作　　者：张萍[1,2] 邵岩飞 林小凤[1] 张梦婷[1] 房盛懿 李丹[1,2] 许超强 陈继承[3] 高碧珍[1,2,4] ZHANG Ping;SHAO Yanfei;LIN Xiaofeng;ZHANG Mengting;FANG Shengyi;LI Dan;XU Chaoqiang;CHEN Jicheng;GAO Bizhen(Fujian University of Chinese Medicine,Fuzhou 350122,China;Key Laboratory of TCM Health Differentiation in Fujian Province,Fujian University of Chinese Medicine,Fuzhou 350122,China;Quanzhou Hospital of Traditional Chinese Medicine,Quanzhou 362000,China;Fujian 2011 Chinese Medicine Health Management Collaborative Innovation Center,Fuzhou 350122,China)

机构地区：[1]福建中医药大学,福建福州350122 [2]福建省中医健康状态辨识重点实验室(福建中医药大学),福建福州350122 [3]泉州市中医院,福建泉州362000 [4]福建省2011中医健康管理协同创新中心,福建福州350122

出　　处：《中国中医药信息杂志》2019年第11期16-21,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81273666、81873234);福建省自然科学基金面上项目(2018J01875);福建省2011中医健康管理协同创新中心资助项目(JG2017004-协同)

摘　　要：目的探讨肥胖基因(FTO)多态性位点rs9939609(A/T)与代谢综合征(MS)痰证病位、病性兼杂规律及理化指标的相关性。方法选择MS痰证患者199例,MS非痰证患者113例,健康人198名,采用证素辨证法分析各证素分布情况;采用SNPscanTM多重SNP分型技术方法检测FTO单核苷酸多态性(SNPs)位点rs9939609的基因型频率、等位基因频率及其与痰证病位、病性兼杂规律的相关性。结果FTO多态性位点rs9939609的3种基因型频率3组比较差异有统计学意义(P<0.01);痰证组、非痰证组基因型AA与AT频率高于健康组(P<0.01)。FTO基因位点rs9939609健康组、痰证组等位基因T频率高于非痰证组(P<0.01)。痰证组中,阴虚的TT基因型频率高于非阴虚(P<0.01),AT基因型的男性比例高于女性(P<0.05),AT基因型的低密度脂蛋白(LDL-C)、肌酐(Cr)明显高于TT基因型(P<0.05,P<0.01)。结论FTO基因与MS形成有关,携带FTO基因多态性位点rs9939609基因型AA、AT和风险等位基因A会增加MS发生风险;FTO多态性位点rs9939609与MS痰证及兼杂病性阴虚的形成相关,FTO是MS痰证易感基因之一;在MS痰证组中,男性更易携带FTO基因多态性位点rs9939609AT基因型;FTO基因多态性位点rs9939609与MS痰证的LDL-C、Cr代谢有关。Objective To investigate the correlation between FTO gene polymorphism locus rs9939609(A/T)and characteristic regularity of location and disease nature as well as physicochemical indexes of metabolic syndrome(MS)with phlegm syndrome.Methods Totally 199 patients with MS phlegm syndrome,113 patients with MS non-phlegm syndrome and 198 healthy people were collected and the distribution of each syndrome element was analyzed by syndrome differentiation.The SNPscanTM multiplex SNP typing technique was used to detect the genotype frequency and allele frequency of FTO single nucleotide polymorphism(SNPs)locus rs9939609.Meanwhile,the correlation between the FTO gene polymorphism locus rs9939609 and characteristic regularity of location as well as disease nature of MS with phlegm syndrome was analyzed.Results There was statistical significance among the genotype frequencies of the three FTO polymorphisms rs9939609 in the three groups(P<0.01);the risk genotype homozygote AA and heterozygote AT in the phlegm syndrome group and non-phlegm syndrome group were significantly higher than those in the healthy group(P<0.01).The FTO allele rs9939609 frequency of allele T in healthy group and phlegm syndrome group was higher than that in non-phlegm syndrome group(P<0.01).In phlegm syndrome group,the frequency of TT genotype in yin deficiency was much higher than that of non-yin deficiency(P<0.01);The proportion of males with AT genotype was higher than that of females(P<0.05);AT genotype LDL-C and Cr were significantly higher than those of TT genotypes(P<0.05,P<0.01).Conclusion FTO gene is related to the formation of MS.Carrying FTO gene polymorphism locus rs9939609 genotype AA and AT and risk allele A may increase the risk of MS;FTO polymorphism locus rs9939609 may be related to the formation of MS phlegm syndrome and yin deficiency of concurrent miscellaneous diseases;In MS phlegm syndrome,males are more likely to carry the FTO gene polymorphism locus rs9939609 AT genotype;FTO gene polymorphism locus rs9939609 may be related to the

关 键 词：代谢综合征 痰证 单核苷酸多态性 证素 FTO基因 

分 类 号：R259.89[医药卫生—中西医结合]