基于转录组的剑麻ARF基因家族的鉴定及表达分析  

Transcriptome-wide Identification and Expression Profiling of the Auxin Response Factor (ARF) Gene Family in Agave.hybrid No.11648

作　　者：鹿志伟 侯晓婉 杨子平[1,2] 张燕梅 李俊峰[1,2] 周文钊 LU Zhiwei;HOU Xiaowan;YANG Ziping;ZHANG Yanmei;LI Junfeng;ZHOU Wenzhao(South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China;Zhanjiang City Key Laboratory for Tropical Crops Genetic Improvement, Zhanjiang, Guangdong 524091, China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]湛江市热带作物遗传改良重点实验室,广东湛江524091

出　　处：《热带作物学报》2019年第10期1972-1978,共7页Chinese Journal of Tropical Crops

基　　金：现代农业产业技术体系建设专项(No.CARS-16);中国热带农业科学院南亚热带作物研究所基本科研业务费专项(No.1630062019021);海南省自然科学基金青年基金项目“剑麻AsMYC2下游抗病关键基因筛选及鉴定”(No.319QN288)

摘　　要：生长素反应因子(AuxinResponseFactors,ARFs)是植物特有的多基因转录因子家族,参与植物生长发育过程的调节。本研究基于转录组数据,通过生物信息学方法鉴定剑麻ARF基因家族成员,同时对其进行相关生物信息学分析。结果表明:在剑麻中共鉴定得到15个ARF基因,并依次命名为AhARF1~15。其编码的蛋白质含有409~1011个氨基酸,分子量约为45.17~112.13ku,等电点为5.17~9.34。亚细胞定位预测结果显示,13个AhARFs定位于细胞核,2个AhARFs定位于叶绿体。保守结构域分析结果表明,AhARFs基因家族有8个成员同时含有B3、ARF和Aux/IAA这3个相对保守的结构域,其他成员仅含有B3和ARF这2个结构域。进化树分析表明,剑麻AhARFs蛋白可分为5个亚家族。15个剑麻AhARFs基因在剑麻紫色卷叶病不同发病时期呈现不同的表达规律。本研究为进一步深入探索剑麻ARF基因家族的功能奠定了重要理论基础。Auxin response factors (ARFs) are plant-specific polygenic transcription factor families involved in the regulation of plant growth and development. In this study, the members of the agave ARF gene family were identified and analyzed by bioinformatics methods, based on transcriptome data. The results showed that 15 ARF genes were identified in agave and named from AhARF 1 to AhARF 15 in turn, its encoded protein varied from 409 to 1011 amino acid residues in length, the molecular weight ranged from 45.17 to 112.13 ku, and the isoelectric point varied from 5.17 to 9.34. Subcellular localization prediction showed that 13 AhARFs were localized in the nucleus and 2 AhARFs were located in the chloroplast. Conservative domain analysis results indicated that there were eight members among the AhARFs gene family which containing three relatively conserved domains, B3, ARF, and Aux/IAA. The other members contained only the two domains, B3 and ARF. Phylogenetic tree analysis indicated that the AhARFs protein could be divided into five subfamilies. The 15 AhARFs genes showed different expression patterns in different stages of agave purple leaf curl disease. This research would lay an important theoretical foundation for further study of the function of the AhARFs gene family.

关 键 词：剑麻 ARF 基因家族 转录组 

分 类 号：S563.8[农业科学—作物学]