一种基于N基因的麻疹病毒实时荧光RT-PCR检测技术  

Study on the N gene-based real-time RT-PCR assay for the detection of measles viru

作　　者：文奇[1] 付倩[1] 宋利军[1] 刘琴[1] 周郡 张艳妮[2] Wen Qi;Fu Qian;Song Lijun;Liu Qin;Zhou Jun;Zhang Yanni(Xinyu Center for Disease Control Prevention,Xinyu 338000,Jiangxi Province, China)

机构地区：[1]新余市疾病预防控制中心,江西新余338000 [2]江西省疾病预防控制中心,江西南昌330029

出　　处：《实验与检验医学》2019年第5期813-815,共3页Experimental and Laboratory Medicine

基　　金：第六批江西省医学学科省市共建计划项目（201412-2）

摘　　要：目的引进一种以检测麻疹病毒N基因为目标的荧光RT-PCR技术并运用到新余市麻疹常规监测工作。方法新荧光RT-PCR与国产的荧光RT-PCR试剂盒、IgM抗体ELISA检测试剂盒分别对新余市2014年所采集麻疹疑似病例咽拭子、血清样本进行检测和结果分析。结果新荧光RT-PCR、国产的荧光RT-PCR试剂盒、ELISA试剂盒的检测阳性率分别为25.53%、23.4%、17.02%。与ELISA相比,RT-PCR具有更好的检测灵敏度,且两种荧光RT-PCR之间的总符合率达到97.87%。结论新引进的方法很敏感,适用于麻疹实验室监测以及疑似病例的快速诊断。Objective To introduce a real-time RT-PCR assay targeting the N gene of measles virus and to apply it in routine surveillance of measles in Xinyu. Methods Collected from measles-suspect cases in Xinyu during the whole 2014,the throat swab specimens were detected for viral RNA by this new technique and some domestic real-time RT-PCR kit respectively,and the serum specimens were tested by ELISA kit for IgM antibody,then the data obtained were analyzed. Results The positive ratios of this new RT-PCR,the domestic RT-PCR kit and the ELISA kit were 25.53%,23.4% and 17.02% respectively. Compared to the ELISA,the two real-time RT-PCR methods had better sensitivity and the total compliance of them was 97.87%. Conclusion This new real-time RT-PCR technique is very sensitive to detect measles virus,and it is suitable for laboratory surveillance of measles and rapid diagnosis of measles-suspect cases.

关 键 词：麻疹病毒 N基因 实时荧光RT-PCR 

分 类 号：R511.1[医药卫生—内科学] Q812[医药卫生—临床医学]