有机氮源添加对嗜热厌氧杆菌产乙醇的影响  

Effect of Organic Nitrogen Source Addition on Ethanol Production by Thermoanaerobacterium aotearoense SCUT27Δldh

作　　者：任梦梦 傅宏鑫 马毅[1] 王菊芳[1] Ren Meng-meng;Fu Hong-xin;Ma Yi;Wang Ju-fang(South China University of Technology, Guangdong Guangzhou 510006)

机构地区：[1]华南理工大学

出　　处：《生物化工》2019年第5期5-9,共5页Biological Chemical Engineering

基　　金：国家自然科学基金资助项目(21676098,21878103)

摘　　要：嗜热厌氧杆菌(Thermoanaerobacterium aotearoense SCUT27Δldh)能利用葡萄糖与木糖发酵,是纤维素乙醇生产的理想菌株。为进一步改善菌株在不同底物(葡萄糖、木糖和混合糖)条件下的乙醇发酵性能,本文探究了有机氮源对嗜热厌氧杆菌发酵产乙醇的影响。结果表明,4 g/L 酵母提取物和2 g/L 胰蛋白胨为最佳的有机氮源组成,在不同底物条件下菌株生物量、底物利用率、乙醇浓度和生产强度与对照相比,分别提升41%~ 109%、50%~ 56%、53%~ 76%和91%~ 111%。此外,本研究还证实了充足的有机氮源供给对于T. aotearoense SCUT27Δldh 的葡萄糖和木糖高效共利用起到重要的作用。Thermoanaerobacterium aotearoense SCUT27Δldh can utilize glucose and xylose, and is an ideal strain for cellulosic ethanol production. In order to improve the fermentation performance of ethanol production using various substrates (glucose, xylose and mixed sugar), the effect of organic nitrogen source addition was investigated. It is showed that the optimum organic nitrogen source composition was 4 g/L yeast extract and 2 g/L tryptone, under which condition the cell density, substrate consumption, ethanol titer and productivity increased by 41%~ 109%、50%~ 56%、 53%~ 76% and 91%~ 111%, respectively. Compared with the control when various substrates were used as the carbon sources. In addition, this study also confirmed that sufficient organic nitrogen source supply played an important role in the glucose and xylose co-utilization for T. aotearoense SCUT27Δldh.

关 键 词：嗜热厌氧杆菌 乙醇 氮源 

分 类 号：Q815[生物学—生物工程]