人工老化对沙葱种子中活性氧与超微弱发光的影响  

Effects of Artificial Aging on ROS and UWL in the Seeds of Allium mongolicum

作　　者：张东[1] 张凤兰[1] 郝丽珍[1] 杨忠仁[1] 王佳琪 赵宏宇[1] 任晓颖 ZHANG Dong;ZHANG Fenglan;HAO Lizhen;YANG Zhongren;WANG Jiaqi;ZHAO Hongyu;REN Xiaoying(College of Horticulture and Plant Protection, Inner Mongolia Agricultural University,Inner Mongolia KeyLaboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement,Hohhot Inner Mongolia 010019,China)

机构地区：[1]内蒙古农业大学园艺与植保学院内蒙古野生特有蔬菜种质资源与种质创新重点实验室

出　　处：《种子》2019年第10期1-5,13,共6页Seed

基　　金：国家自然科学基金(31760081,31260474);内蒙古自然科学基金(2015 MS 0362);内蒙古农业大学动植物品种选育专项,公益性行业(农业)科研专项(201203004)

摘　　要：以人工老化和Ce^3+浸种后再人工老化的沙葱种子为试材,对种子活力、电导率、呼吸速率、活性氧和超微弱发光等指标进行了研究,探讨Ce3+浸种对沙葱老化种子活性氧与超微弱发光的影响。结果表明,Ce^3+1处理下,沙葱种子的发芽率、活力指数和胚根长分别比ck1高4%、0.042和1.64cm,表明Ce^3+有助于提高种子活力及促进胚根的伸长;在老化程度较低的情况下,Ce^3+前处理对沙葱种子电解质外渗有一定缓解作用;活性氧在老化15h前,Ce^3+处理的超氧阴离子产生速率低于ck;Ce^3+比ck的超微弱发光强度高,Ce^3+1和Ce^3+0的超微弱发光强度差异不显著,Ce^3+0显著高于Ce^3+2、Ce^3+3和Ce^3+4的超微弱发光强度。超微弱发光与胚根长、超氧阴离子产生速率和过氧化氢含量呈极显著正相关,表明超微弱发光强度随活性氧的变化而变化。在种子的加速老化过程中,Ce3+处理可调控活性氧的增加,减缓老化对细胞损伤,维持细胞功能稳定性。In order to explore the effects of cerium (Ce ^3+)-soaking seed on physiological indicators including reactive oxygen and ultra-weak light emission,we detected the seed activity,electrical conductivity,respiration rate,reactive oxygen and ultra-weak light emission of Allium mongolicum seeds treated with artificial aging and artificial aging after cerium (Ce^ 3+)-soaking seeds respectively.The results showed that the germination rate,vigor index and radicle length of A. mongolicum seeds under Ce ^3+ 1 treatment were 4%,0.042 and 1.64 cm higher than ck 1,indicating that Ce 3+ could improve seed vigor and promote radicle elongation.Under the condition of low aging degree,pre-treatment of Ce ^3+ could alleviate electrolyte exosmosis of shallot seeds to some extent.Additionally,Before aging 15 hours,production rate of the superoxide anion in Ce ^3+ treatment seeds was lower than that of ck,while ultra-weak luminescence intensity in Ce ^3+ treatment seeds was higher than that of ck.There was no significant difference in the ultra-weak luminescence intensity between Ce ^3+ 1 and Ce ^3+ 0.And the ultra-weak luminescence intensity of Ce^ 3+ 0 treatment seeds was significantly higher than that in Ce^ 3+ 2,Ce ^3+ 3 and Ce^3+ 4.The ultra-weak luminescence intensity was significantly positively correlated with radicle length,anion production rate and hydrogen peroxide content,indicating that the ultra-weak luminescence intensity changed with the change of reactive oxygen species.In a word,in the accelerated aging process of seeds,Ce^3+ treatment could regulate the increase of reactive oxygen species,slow down the damage of aging to cells,and maintain the stability of cell function.

关 键 词：沙葱种子 人工老化 稀土铈 活性氧 超微弱发光 

分 类 号：S633.9[农业科学—蔬菜学]