利用基因芯片技术研究葱白提取物对血脂的调控基因  

Regulatory gene of allium fistulosum extract on blood lipid by using gene chip technology

作　　者：冯云霞[1] 张介眉[1] 谢沛霖[1] 郝建军[1] 朱旭[1] FENG Yunxia;ZHANG Jiemei;XIE Peilin;HAO Jianjun;ZHU Xu(Wuhan Municipal Hospital of Integrated Traditional Chinese and Western Medicine,Wuhan,Hubei 430000,China)

机构地区：[1]武汉市中西医结合医院

出　　处：《重庆医学》2019年第20期3511-3515,共5页Chongqing medicine

基　　金：全国名老中医传承工作室建设项目(国中医药人教发[2011]41号);湖北省武汉市卫生和计划生育委员会科研项目(WZ19Q01)

摘　　要：目的利用基因芯片技术探讨葱白提取物调节血脂的可能机制。方法利用高脂饲料法建立高脂血症模型,葱白提取物组给予葱白提取物灌胃6周,造模成功后分别提取模型组和葱白提取物组肝脏组织的总RNA,标记后与OneArray大鼠基因芯片杂交,洗脱、染色、扫描芯片,GCOS软件分析和处理数据;运用实时荧光定量PCR技术对Fgf21、Scap基因进行验证。结果按照表达差异倍数大于等于2,P<0.05的筛选条件,筛选出高脂血症模型组和葱白提取物组差异基因有58个,其中表达上调的基因16个,表达下调的基因42个。经PCR验证:与模型组相比,葱白提取物可以明显上调Fgf21的表达(P<0.05),而对Scap的表达影响不大,这与基因芯片结果一致。结论基因芯片技术是一高效筛选葱白提取物调节血脂相关基因的方法,Cpt1b、Cidea、Lcn2、Fgf21、Scd等基因可能与葱白提取物调节血脂机制相关。Objective To investigate the possible mechanisms of allium fistulosum extract for regulating blood lipid by using the gene chip technology. Methods The hyperlipidemia model was established by using the high fat diet method.The allium fistulosum extract group was gavaged by allium fistulosum extract for 6 weeks.After constructing the mode,total RNA was extracted from the model and allium fistulosum extract groups,after labeling,which were hybridized with OneArray rat microarray for conducting elution,staining,scanning chip,GCOS software analysis and processing the data;the real-time PCR technique was used to verify the Fgf21,Scap gene. Results Fifty-eight differential genes were screened out from the model group and allium fistulosum extract group according to the screening conditions of the expression difference fold≥2 and P <0.05.Among them,16 genes expressed the upregulation and 42 genes expressed the downregulation.By PCR verification,compared with the model group,allium fistulosum could significantly up-regulate the expression of Fgf21( P <0.05),but had little effect on the expression of Scap,which was consistent with the gene chip results. Conclusion The gene chip technology is a highly efficient method for creening allium fistulosum extract for regulating blood lipid related genes,and Cpt1b,Cidea,Lcn2,Scd,Fgf21 and other genes may be related with allium fistulosum extract regulating blood lipid mechanisms.

关 键 词：高脂血症 葱白提取物 基因芯片 差异基因 

分 类 号：R285.5[医药卫生—中药学]