瑶药糯米风醇提物的化学成分研究  

Study on Chemical Constituents of Ethanol Extract from Yao Medicine Cissampelopsis spelaeicola

作　　者：梁爽 马雯芳 梁可文 赵立春 邓刚 谢冬养 LIANG Shuang;MA Wenfang;LIANG Kewen;ZHAO Lichun;DENG Gang;XIE Dongyang(College of Pharmacy,Guangxi University of TCM,Nanning 530001,China;Guangxi Jianfeng Pharmaceutical Co.,Ltd.,Nanning 530104,China;Guilin Niutai Biological Technology Co.,Ltd.,Guangxi Guilin 541004,China)

机构地区：[1]广西中医药大学药学院,南宁530001 [2]广西健丰药业有限公司,南宁530104 [3]桂林纽泰生物科技有限公司,广西桂林541004

出　　处：《中国药房》2019年第20期2835-2839,共5页China Pharmacy

基　　金：广西自然科学基金资助项目（No.2016GXNSFAA380066）;广西壮族自治区科学技术厅重点实验室项目（No.桂科基字〔2014〕32号）;广西协同创新中心建设项目（No.桂教科研〔2014〕13号）;广西高校壮医方药基础与应用研究重点实验室科研课题（No.桂教科研〔2016〕6号zyfy201605）;广西壮族自治区食品药品监督管理局《广西壮族自治区瑶药材质量标准（第二卷）》质量评价与标准研究项目

摘　　要：目的:研究瑶药糯米风醇提物的化学成分。方法:采用硅胶柱、SephadexLH-20凝胶柱、AB-8大孔树脂柱等对糯米风75%乙醇提取物的石油醚、乙酸乙酯和正丁醇部位进行分离纯化,根据理化性质和波谱(质谱、氢谱、碳谱)数据分析鉴定所得化合物的结构。结果与结论:从糯米风75%乙醇提取物中共分离鉴定出12个化合物,其中从石油醚部位获得β-谷甾醇(Ⅰ)、豆甾醇(Ⅱ),从乙酸乙酯部位获得对羟基苯甲酸(Ⅲ)、β-胡萝卜苷(Ⅳ)、原儿茶酸(Ⅴ)、6β-羟基-艾里莫芬-7(11)-烯-12,8β-内酯(Ⅵ)、10β-羟基-7(11)-烯-8,12-艾里莫酚内酯(Ⅶ)、10β-羟基-7(11),8(9)-二烯-8,12-艾里莫酚内酯(Ⅷ)、槲皮素(Ⅸ)、金丝桃苷(Ⅹ)、4α-hydroxy-eudesman-11-ene(Ⅺ),从正丁醇部位获得槲皮素-3-O-洋槐糖苷(Ⅻ)。化合物Ⅰ~Ⅻ均为首次从该植物中分离得到。本研究可为糯米风的药理活性研究提供物质基础。OBJECTIVE:To study the chemical constituents of ethanol extract from Yao medicine Cissampelopsis spelaeicola. METHODS:The petroleum ether,ethyl acetate and n-butanol fraction from 75% ethanol extract of C.spelaeicola were isolated and purified by silica gel,SephadexLH-20 gel column and AB-8 macroporous resin column,etc.The structures of the compounds were analyzed and identified by physicochemical properties and spectral data (mass spectrometry,hydrogen spectrum,carbon spectrum).RESULTS & CONCLUSIONS:Twelve compounds were isolated and identified from 75% ethanol extract of C. spelaeicola.β-sitosterol(Ⅰ) and Stigmasterol(Ⅱ) were isolated from petroleum ether fraction;p-hydroxybenzoic acid(Ⅲ),β-daucossterol(Ⅳ),protocatechuic acid(Ⅴ),6β-hydroxyeremophi-7(11)-en-12,8β-olide(Ⅵ),10β-hydroxyeremophil-7(11)-en-8, 12-olide(Ⅶ),10 β-hydroxyeremophi-7(11),8(9)-dien-8,12-olide(Ⅷ),Quercetin(Ⅸ),Hyperin(Ⅹ) and 4 α-hydroxyeudesman- 11-ene(Ⅺ) were isolated from ethyl acetate fraction;quercetin-3-O-robinobioside(Ⅻ) was isolated from n-butanol fraction.Compounds Ⅰ-Ⅻ are isolated from C.spelaeicola for the first time.The study can lay material foundation for activity stady of C.spelaeicola.

关 键 词：糯米风 化学成分 分离鉴定 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]