决明子蒽醌苷对非酒精性脂肪肝病大鼠肝脏组织中SREBP-1c和PPARα表达的影响  

The affection of cassia glycosides on SREBP-1c and PPARα in liver of nonalcoholic fatty liver disease rats

作　　者：李玉晶 侯伟 武俊紫 宋波[1] 陈文慧[1] LI Yujing;HOU Wei;WU Junzi;SONG Bo;CHEN Wenhui(College of Basic Medicine ,Yunnan University of TCM ,Kunming 650504,China;Central Laboratory ,Environmental Monitoring Center of Kunming ,Kunming 640288,China)

机构地区：[1]云南中医学院基础医学院,云南昆明650504 [2]昆明卫生职业学院,云南昆明650600

出　　处：《西部医学》2019年第10期1511-1516,共6页Medical Journal of West China

基　　金：国家自然科学基金地区基金项目(81160492)

摘　　要：目的探讨分析决明子蒽醌苷对脂代谢相关基因固醇调节元件结合蛋白-1C(SREBP-1c)和过氧化物酶体增殖物激活受体ɑ(PPARα)表达的影响。方法将60只SD大鼠随机分为正常组、模型组、多烯磷脂酰胆碱干预组、决明子蒽醌苷低、中和高剂量治疗组,每组10只,除正常组外,其余5组给予高脂高糖饲料8周后构建NAFLD模型,给予相应药物治疗6周,称重后处死大鼠,采集血液和肝脏标本,HE染色检测肝脏病理学,计算肝指数、测定ALP、Tbil、Dbil、HDL-C和LDL-C,实时荧光定量PCR技术和Western Blot技术测定肝组织SREBP-1c和PPARα蛋白和mRNA表达。结果与正常组相比,模型组出现明显的脂肪空泡,而多烯磷脂酰胆碱和决明子蒽醌苷则可以明显降低其脂肪空泡数量;与正常组相比,模型组肝指数、ALP、Tbil、Dbil和LDL-C明显升高(P<0.05),相较于模型组多烯磷脂酰胆碱和决明子蒽醌苷上述指标明显降低(P<0.05),此外决明子蒽醌苷高剂量组肝指数和ALP明显低于多烯磷脂酰胆碱治疗组(P<0.05),决明子蒽醌苷三个剂量组Tbil和Dbil,均明显低于多烯磷脂酰胆碱治疗组(P<0.05),而LDL-C多烯磷脂酰胆碱和决明子蒽醌苷三个剂量组没有差异。与正常组相比,模型组HDL-C明显降低(P<0.05),多烯磷脂酰胆碱和决明子蒽醌苷均可以升高HDL-C(P<0.05),此外多烯磷脂酰胆碱三个治疗组与多烯磷脂酰胆碱治疗组没有明显差异;多烯磷脂酰胆碱和决明子蒽醌苷治疗后SREBP-1c明显降低(P<0.05),PPARα明显升高(P<0.05),此外SREBP-1c和PPARα蛋白表达,决明子蒽醌苷三个治疗组均明显低于多烯磷脂酰胆碱干预组(P<0.05)。结论相较于临床中常用的药物多烯磷脂酰胆碱,决明子蒽醌苷同样可以明显的改善肝功能,调节血脂,这与决明子蒽醌苷可以更多的抑制肝脏中SREBP-1c表达有关。Objective To investigate the effect of cassia glycosides on sterol regulatory element binding protein-1 c(SREBP-1 c) and peroxisome proliferators-activated receptor ɑ(PPARα) in nonalcoholic fatty liver disease(NAFLD) rats. Methods 60 SD rats were randomly divided into the normal group, model group, positive drug polyene phosphatidylcholine group, cassia glycosides low, middle and high dose groups, n=10. Except the normal group, the others were receive a 8 weeks of diet with high fat and high sugar to build the NAFLD model, after the NAFLD model was successfully constructed, those rats were received a 6 weeks treatment, then the rats were sacrificed. Serum and liver were collected. Hepatic pathology was detected by HE staining, liver index was calculated, ALP, Tbil, Dbil, HDL-C and LDL-C were measured. Real-time PCR and Western Blot were used to determine the expression of SREBP-1 c and PPARα protein and mRNA in liver tissue. Results Compared with the normal group, the results of HE staining showed obvious fat vacuoles the model group, while the polyene phosphatidylcholine and cassia glycoside could significantly reduce the number of fat vacuoles. Compared with the normal group, the liver index, ALP, Tbil, Dbil, and LDL-C were significantly increased in model group(P<0.05). Compared with the model group, those indexes in the polyene phosphatidylcholine and cassia glycoside groups were significantly decreased(P<0.05). The liver index and ALP in the high dose of cassia glycoside group were significantly lower than those in the polyene phosphatidylcholine group(P<0.05). Tbil and Dbil in the three dose groups of cassia glycoside group were significantly lower than those in the polyene phosphatidylcholine group(P<0.05). However, for LDL-C there was no difference between the polyene phosphatidylcholine and cassia glucosides group. Compared with the normal group, the HDL-C in the model group was significantly lower(P<0.05), polyene phosphatidylcholine and cassia glucosides could increase HDL-C(P<0.05), there were no s

关 键 词：决明子蒽醌苷 非酒精性脂肪肝 固醇调节元件结合蛋白-1C 过氧化物酶体增殖物激活受体ɑ 

分 类 号：R285.5[医药卫生—中药学]