烷化甘油磷酸合酶在异丙肾上腺素诱导的心肌肥厚中的作用  

Role of the alkylglycerone phosphate synthase in isoproterenol-induced cardiac hypertrophy

作　　者：刘艺洁[1] 费乔曼 曹冰雁 邱曼曼 黄欢 宋佳鑫 杨冰[1] 张玲[1] hypertrophy Liu Yijie;Fei Qiaoman;Cao Bingyan;Qiu Manman;Huang Huan;Song Jiaxin;Yang Bing;Zhang Ling(School of Basic Medical Sciences,Tianjin Medical University,Tianjin 300070,China;School of Pharmacy,TianjinMedical University,Tianjin 300070,China)

机构地区：[1]天津医科大学基础医学院,300070 [2]天津医科大学药学院,300070

出　　处：《国际生物医学工程杂志》2019年第4期301-306,共6页International Journal of Biomedical Engineering

基　　金：天津市自然科学基金(15JCYBJC25400,16JCQNJCl2100);天津市高等学校科技发展基金计划项目(20130103);教育部留学回国人员科研启动基金资助项目.

摘　　要：目的探讨烷化甘油磷酸合酶(AGPS)在异丙肾上腺素(ISO)诱导的大鼠心肌肥厚过程中的作用.方法采用ISO腹腔注射法构建大鼠病理性心肌肥厚模型.选取健康Sprague-Dawley大鼠(120~150 g)12只,采用抽签法将大鼠随机分为ISO处理组和正常对照组,每组6只.ISO处理组大鼠每天腹腔注射ISO(3 mg/kg),正常对照组大鼠腹腔注射等体积的生理盐水,连续注射2周.通过超声心动检测大鼠左室舒张期内径、左室后壁厚度、左室射血分数、左室短轴缩短率和左室质量的变化,苏木素-伊红染色检测大鼠心肌细胞横截面积的大小,蛋白质印迹法和实时荧光定量PCR检测大鼠心肌组织中3种肥厚因子[心房利钠肽(ANP)、肌球蛋白轻链-2V(MLC-2V)、α-肌球蛋白重链(α-MHC)]和AGPS的mRNA和蛋白表达.结果超声心动检测结果表明大鼠心肌肥厚模型构建成功.苏木素-伊红染色结果显示,ISO处理组大鼠心肌横截面积明显大于正常对照组.蛋白质印迹法和实时荧光定量PCR结果表明:与正常对照组相比,ISO处理组大鼠心肌组织中3种肥厚因子及AGPS的mRNA和蛋白相对表达量均上调,差异均具有统计学意义(均P<0.05).结论构建的大鼠病理性心肌肥厚模型中AGPS表达上调,为进一步研究AGPS在病理性心肌肥厚发病机制中的作用提供了理论依据.Objective To research the effect of alkylation of glycerol phosphate synthase (AGPS) in isoproterenol (ISO) induced rat cardiac hypertrophy. Methods The pathological cardiac hypertrophy rat model was constructed by ISO intraperitoneal injection. Twelve healthy Sprague-Dawley rats (120~150 g) were divided into ISO group and control group randomly. In the ISO group, rats were injected with ISO (3 mg/kg) per day for two consecutive weeks. In the control group, rats were injected with normal saline (3 mg/kg) per day for two consecutive weeks. Changes of left ventricular diastolic diameter, left ventricular posterior wall thickness, left ventricular ejection fraction, left ventricular short-axis shortening rate and left ventricular mass were detected by echocardiography. The cross-sectional area of myocardial cells in rats was measured by hematoxylin-eosin staining. The expression of hypertrophic factors [atrial natriuretic peptide (ANP), myosin light chain-2V (MLC-2V),α-myosin heavy chain (α-MHC)] and AGPS were detected by Western Blot and real-time quantitative PCR (qPCR). Results The results of echocardiography showed that the cardiac hypertrophy rat model was successfully constructed. The results of hematoxylin-eosin staining showed that the myocardial cross-sectional area in the ISO group was significantly larger than that of the control group. The Western Blot and qPCR results indicated that the relative expression of protein and mRNA of hypertrophic factor and AGPS in the ISO group were both up-regulated comparing with that of the control group, and the differences were statistical significance (all P<0.05). Conclusions The rat model of pathological cardiac hypertrophy with up-regulated AGPS expression was successfully constructed providing a theoretical basis for further study on the role of AGPS in pathogenesis of pathological cardiac hypertrophy.

关 键 词：心肌病 肥厚性 异丙肾上腺素 烷化甘油磷酸合酶 

分 类 号：R542.2[医药卫生—心血管疾病]