重组人CREG-Fc融合蛋白对小鼠血管重塑作用研究  

Effect of recombinant human CREG-Fc fusion protein on vascular remodeling in mice

作　　者：田孝祥 刘丹 王硕 宋海旭[1] 张艳 赵晓杰 罗安德 闫承慧 TIAN Xiao-xiang;LIU Dan;WANG Shuo;SONG Hai-xu;ZHANG Yan;ZHAO Xiao-jie;LUO An-de;YAN Cheng-hui(Department of Cardiology,General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]北部战区总医院心血管内科,辽宁沈阳110016 [2]中国医科大学附属盛京医院心内科,辽宁沈阳110004 [3]先声生物医药科技有限公司,江苏南京210000

出　　处：《临床军医杂志》2019年第10期1070-1072,共3页Clinical Journal of Medical Officers

基　　金：国家自然科学基金(81570767);沈阳市科技计划项目(19-112-4-052)

摘　　要：目的探讨外源性重组人CREG-Fc融合蛋白对血管紧张素Ⅱ(AngⅡ)诱导的小鼠血管重塑作用。方法选取20只8周龄雄性C57 BL/6小鼠为研究对象。将小鼠随机分为对照组、AngⅡ处理组、AngⅡ+CREG-His蛋白治疗组及AngⅡ+CREG-Fc蛋白治疗组,每组各5只。AngⅡ及蛋白均通过皮下埋置微渗透泵方式持续给药28 d。通过尾套法测定不同时间点各组小鼠心率及血压。HE染色检测主动脉厚度,Masson染色检测主动脉纤维化情况,评价主动脉重塑情况。结果各组小鼠不同时间点心率比较,差异无统计学意义(P>0.05)。第2、3、7、14、21、28天AngⅡ处理的3组小鼠的收缩压均显著高于对照组,差异均有统计学意义(P<0.05)。AngⅡ+CREG-His组第7、14、21、28天收缩压显著低于单纯AngⅡ处理组,两组比较,差异有统计学意义(P<0.05)。AngⅡ+CREG-Fc组第21、28天收缩压显著低于AngⅡ+CREG-His组,两组比较,差异有统计学意义(P<0.05)。结论外源性CREG-Fc融合蛋白可对抗AngⅡ诱导的血压升高及血管重构,并且后期治疗效果优于CREG-His融合蛋白。Objective To investigate the effect of exogeneous recombinant human CREG-Fc fusion protein on AngiotensinⅡ(AngⅡ)induced vascular remodeling in mice.Methods Twenty 8-week-old male C57 BL/6 mice were selected as the study subjects.Mice were randomly divided into the control group,AngⅡtreatment group,AngⅡ+CREG-His protein in treatment group and AngⅡ+CREG-Fc protein in treatment group,each group with 5.AngⅡand protein through the subcutaneous embedded micro osmotic pump continuous dosing 28 days.Heart rate and blood pressure were measured by caudate cuff method.Aortic thickness was detected by HE staining,and aortic fibrosis was detected by Masson staining,and aortic remodeling was evaluated.Results There was no significant difference in heart rate at different time points(P>0.05).The 2,3,7,14,21 and 28 days AngⅡprocessing of three groups of systolic blood pressure in mice were significantly higher than the control group,and the difference had statistical significance(P<0.05).AngⅡ+CREG-His group 7,14,21 and 28 days systolic blood pressure was significantly lower than pure AngⅡtreatment group,two groups of comparison(P<0.05).AngⅡ+CREG-Fc group 21,28 days,systolic blood pressure was significantly lower than AngⅡ+CREG-His group(P<0.05).Conclusion Exogenous CREG-Fc fusion protein can be against AngⅡinduced high blood pressure and vascular remodeling,and later treatment effect is better than that of CREG-His fusion protein.

关 键 词：CREG-Fc 融合蛋白 血管重塑 

分 类 号：R543.5[医药卫生—心血管疾病]