电针对颈部切口痛大鼠颈部脊髓中炎性细胞因子的影响  

Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisional neck pain rats

作　　者：王俊英[1] 高永辉[1] 乔丽娜[1] 张金铃[1] 荣培晶[1] 刘俊岭[1] WANG Jun-ying;GAO Yong-hui;QIAO Li-na;ZHANG Jin-ling;RONG Pei-jing;LIU Jun-ling(Institute of Acupuncture an dMoxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院针灸研究所

出　　处：《针刺研究》2019年第10期703-708,共6页Acupuncture Research

基　　金：国家重点基础研究发展计划“973”中医专项(No.2013CB531904);国家自然科学基金项目(No.81202762);中央级公益性科研院所基本科研业务费专项资金资助(No.ZZ11-099)

摘　　要：目的:观察电针对颈部切口痛大鼠颈部脊髓中肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10和白细胞介素-4(IL-4)的影响,探讨电针对切口痛大鼠镇痛作用的抗炎机制。方法:SD大鼠随机分为正常对照组、模型组、扶突组和足三里-阳陵泉组,每组21只。采用颈部甲状腺区域切口复制切口痛模型,电针组分别在手术时,术后20、44 h时电针"扶突"或"足三里"-"阳陵泉"治疗,30 min/次。采用甩尾测痛仪测定大鼠颈部甲状腺区域热痛阈值,采用免疫荧光法观察颈部脊髓背角TNF-α、IL-10表达变化,采用实时荧光定量PCR法观察TNF-α、IL-10、IL-4、IL-4R mRNA的变化。结果:与正常对照组比较,模型组各时点颈部切口区域痛阈值降低(P<0.05)。术后24 h,与模型组比较,扶突组颈部切口区域痛阈值明显升高(P<0.05)。术后24 h,与正常对照组比较,模型组TNF-α(主要表达在星形胶质细胞上)、IL-10的表达升高(P<0.05),IL-4R mRNA的表达降低(P<0.05);与模型组比较,扶突组TNF-α、IL-10的表达显著降低(P<0.05),IL-4和IL-4R mRNA的表达显著升高(P<0.05),足三里-阳陵泉组TNF-αmRNA、IL-10 mRNA、IL-10的表达均显著降低(P<0.05)。结论:电针"扶突"穴对颈部切口痛的镇痛效应,可能与抑制颈部脊髓中TNF-α、促进IL-4/IL-4R的表达有关,并且电针"扶突"镇痛效应优于"足三里"-"阳陵泉"。Objective To observe the effect of electroacupuncture(EA)on incisional pain and expression of tumor necrosis factor-α(TNF-α),interleukin-10(IL-10)and interleukin-4(IL-4)of cervical dorsal part of spinal cord in rats with incisional neck pain,so as to explore its analgesic mechanisms.Methods Eighty-four male SD rats were randomly divided into normal control,model,EA-Futu(LI18)and EA-Zusanli(ST36)-Yanglingquan(GB34,EA-ST36-GB34)groups(n=21 in each group).The incisional neck pain model was established by making a longitudinal incision along the bilateral cervical thyroid regions and repeated mechanical separation stimulation.For rats of the EA groups,EA(2 Hz/100 Hz,1 m A)was applied to bilateral LI18 or ST36-GB34 for 30 min/time during the surgery,and 20 and 44 h after surgery,respectively.The thermal pain threshold(TPT)of the incisional region was detected.The immunoactivity of TNF-αand IL-10 of the dorsal portion of the cervical spinal cord(C2-C5)was detected by immunofluorescence,and the expression of TNF-α,IL-10,IL-4 and IL-4 receptor(IL-4 R)m RNAs was determined by quantitative real-time PCR.Results Compared with the normal group,the TPT of the incisional area was significantly decreased at 4,24 and 48 h after neckincision(P<0.05),the levels of TNF-αm RNA,IL-10 m RNA and TNF-αIL-10 immunoactivity at 24 h were remarkably increased(P<0.05),and the expression of IL-4 R m RNA was considerably decreased at 24 h in the model group(P<0.05).Following EA intervention,the TPT,and expression levels of IL-4 m RNA and IL-4 R m RNA were significantly increased at 24 h after surgery in the EA-LI18 group relevant to the model group(P<0.05),while the expression level of TNF-α(coexpressed with microgliacytes)in the EA-LI18 group,and TNF-αm RNA expression at 24 h in both EA-LI18 and EA-ST36-GB34 groups,as well as the expression of IL-10 and IL-10 m RNA at 24 h in both EA-LI18 and EA-ST36-GB34 groups were significantly decreased(P<0.05).The effect of EA LI18 was significantly superior to that of EA ST36-GB34 in up-regulati

关 键 词：电针 切口痛 脊髓背角 炎性细胞因子 

分 类 号：R245.97[医药卫生—针灸推拿学]