电针对骶上脊髓横断所致膀胱逼尿肌反射亢进大鼠脊髓Wnt/β-catenin信号通路相关蛋白表达的影响  

Effect of electroacupuncture on urodynamics and expression of Wnt-1,β-catenin,and Ngn1 in the spinal cord in rats with bladder detrusor hyperreflexia due to supersacral spinal cord transection

作　　者：邓悦宁 周达岸 徐笑梅 刘磊 聂晓娇 马贤德[2] 刘树权[3] 吕欣 DENG Yue-ning;ZHOU Da-an;XU Xiao-mei;LIU Lei;NIE Xiao-jiao;MA Xian-de;LIU Shu-quan;Lü Xin(TheThird Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China;Teaching Experiment Center,Liaoning University of Traditional Chinese Medicine,Shenyang 116600;Shenyang Second Hospital of Traditional Chinese Medicine,Shenyang 110100)

机构地区：[1]锦州医科大学附属第三医院,辽宁锦州121000 [2]辽宁中医药大学教学实验中心,沈阳116600 [3]沈阳市第二中医医院,沈阳110100

出　　处：《针刺研究》2019年第10期722-728,共7页Acupuncture Research

基　　金：国家自然科学基金项目(No.81673925);辽宁省自然科学基金指导计划项目(No.20180551081)

摘　　要：目的:观察电针"大椎""次髎"穴对骶上脊髓横断后膀胱逼尿肌反射亢进大鼠的干预作用,探讨电针通过调控Wnt-1、β-连环蛋白(β-catenin)和神经基因蛋白1(Ngn1)表达而改善膀胱逼尿肌反射亢进大鼠排尿功能的作用机制。方法:雌性SD大鼠随机分为假手术组、模型对照组、电针组、电针对照组,每组12只。采用胸(T)10脊髓横断制备膀胱逼尿肌反射亢进模型,挤压膀胱排尿法辅助排尿。电针组予"大椎""次髎"穴电针干预;电针对照组于"大椎""次髎"穴区旁开(左右交替)1 cm处进行电针干预,每次20 min,每日1次,连续1周。通过脊髓损伤行为学(BBB)评分评价术后大鼠运动功能;通过尿流动力学判断模型大鼠的排尿功能;Western blot法和免疫组织化学法检测脊髓组织中Wnt-1和β-catenin蛋白表达水平;免疫荧光法检测脊髓组织中Ngn1蛋白表达水平。结果:与假手术组比较,模型对照组大鼠BBB评分显著下降(P<0.01);电针组大鼠BBB评分显著高于模型对照组和电针对照组(P<0.01)。与假手术组比较,模型对照组大鼠膀胱基础压力、最大压力和漏尿点压力升高(P<0.01),膀胱最大容量和顺应性下降(P<0.01);与模型对照组比较,电针组大鼠膀胱基础压力、最大压力和漏尿点压力下降(P<0.01),膀胱最大容量和顺应性升高(P<0.01,P<0.05);与电针组比较,电针对照组大鼠膀胱基础压力、最大压力和漏尿点压力升高(P<0.01),膀胱最大容量和顺应性下降(P<0.01,P<0.05)。与假手术组比较,模型对照组大鼠脊髓组织中Wnt-1、β-catenin蛋白表达水平升高(P<0.05,P<0.01),Ngn1蛋白表达水平下降(P<0.01);与模型对照组比较,电针组大鼠脊髓组织中Wnt-1、β-catenin和Ngn1蛋白表达水平升高(P<0.01);与电针组比较,电针对照组大鼠脊髓组织中Wnt-1、β-catenin和Ngn1蛋白表达水平下降(P<0.01)。结论:电针"大椎""次髎"穴对骶上脊髓横断所致膀胱逼尿肌反射亢进大鼠排尿�Objective To investigate the effect of electroacupuncture(EA)at"Dazhui"(GV14)and"Ciliao"(BL32)on rats with bladder detrusor hyperreflexia(DH)after supersacral spinal cord transection,as well as the mechanism of EA in improving the urinary function by regulating the expression of Wnt-1,β-catenin and Neurogenin 1(Ngn1).Methods A total of 48 female Sprague-Dawley rats were randomly divided into sham-operation group,model control group,EA group,and EA control group,with 12 rats in each group.T10 spinal cord transection(SCT)was performed by surgery.The Basso,Beattie and Bresnahan(BBB)score was used to evaluate the motor function of SCT rat,and the Crede technique was used to assist urination.After the urine volume became stable,the urodynamic test was used to determine whether a rat model of DH was successfully established.The rats in the EA group were given EA at GV14 and BL32,and those in the EA control group were given EA(10 Hz/50 Hz,20 min)at the acupuncture points at 1 cm next to GV14 and BL32 at both sides alternatively.EA was performed once a day for one week.Urodynamic parameters were used to evaluate urinary function.Western blot and immunohistochemistry were used to measure the expression of Wnt-1 andβ-catenin in the spinal cord,and immunofluorescence assay was used to measure the expression of Ngn1 in the spinal cord.Results The BBB score of the model control group significantly decreased compared with that of the sham-operation group(P<0.01),and the EA group was significantly higher than the model control group and the EA control group.Compared with the sham-operation group,the model control group had significant increases in bladder base pressure,maximum pressure,and leak point pressure(P<0.01)and significant reductions in maximum bladder capacity and compliance(P<0.01).Compared with the model control group,the EA group had significant reductions in bladder base pressure,maximum pressure,and leak point pressure(P<0.01)and significant increases in maximum bladder capacity and compliance(P<0.01,P<0.05).Co

关 键 词：电针 骶上脊髓横断 逼尿肌反射亢进 WNT-1 β-连环蛋白 神经基因蛋白1 

分 类 号：R245.81[医药卫生—针灸推拿学]