电针不同穴位对多囊卵巢综合征大鼠卵泡发育及相关因素的影响  

Effect of electroacupuncture at different acupoints on follicle development and related factors in serum and ovary tissues of PCOS rats

作　　者：徐鸽 张安冬 王茜[3] 刘建党 冯戟玮 陈跃来 XU Ge;ZHANG An-dong;WANG Xi;LIU Jian-dang;FENG Ji-wei;CHEN Yue-lai(Teaching and Experimental Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China;Graduade School,Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437)

机构地区：[1]上海中医药大学教学实验中心,上海201210 [2]上海中医药大学研究生院,上海201210 [3]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《针刺研究》2019年第10期740-746,751,共8页Acupuncture Research

基　　金：上海市博士点基金项目(No.B201407)

摘　　要：目的:观察电针不同穴位对多囊卵巢综合征(PCOS)大鼠卵泡发育、促性腺激素、促性腺激素相关受体表达及卵巢抗苗勒氏管激素(AMH)、抑制素B(INHB)水平的影响,探讨电针治疗PCOS的可能机制。方法:雌性SD大鼠按随机数字表法分为对照组、模型组、足三里组、关元组、三阴交组、综合组,每组10只。除对照组外,其余各组大鼠用来曲唑连续灌胃21 d诱导PCOS模型,足三里组、关元组、三阴交组分别选取"足三里""关元""三阴交"进行电针干预,综合组3穴同时进行电针干预,均干预14 d。干预结束后用HE染色法进行大鼠卵泡计数,用酶联免疫吸附法检测血清促黄体生成素(LH)、促卵泡生成素(FSH)、INHB、AMH水平,用免疫组织化学法检测卵巢FSH受体(FSHR)和LH受体(LHR)的表达。结果:与对照组比较,模型组大鼠的生长期卵泡数及血清LH、LH/FSH、AMH、INHB水平均明显升高(P<0.05,P<0.01),而FSH水平、早期卵泡中FSHR和LHR表达明显降低(P<0.01,P<0.05)。与模型组比较,三阴交、关元和综合组的生长期卵泡数明显下降(P<0.05,P<0.01),关元、足三里和综合组INHB水平明显下降(P<0.01),4个电针组的LH、LH/FSH、AMH水平均明显下降(P<0.01),而4个电针组的FSH均明显升高(P<0.01),足三里组大鼠早期卵泡FSHR阳性表达明显升高(P<0.05),足三里组和关元组大鼠早期和晚期卵泡LHR阳性表达均明显升高(P<0.01,P<0.05)。结论:电针"关元""三阴交""足三里"及电针3穴均可减少PCOS大鼠生长期的卵泡数量并调节大鼠性腺激素的表达水平。在上调血清FSH和下调LH/FSH比值、AMH、INHB及改善促性腺激素受体表达方面,电针"关元"和电针"足三里"的作用明显优于电针"三阴交";而在下调LH水平方面,电针"三阴交"的作用明显优于电针"关元";但3个穴位之间没有协同作用。Objective To observe the effect of electroacupuncture(EA)at different acupoints on follicle development,expression of gonadotropins and their receptors and anti-Müllerian hormone(AMH),inhibin B(INHB)in polycystic ovarian syndrome(PCOS)rats,so as to explore its mechanisms underlying improvement of PCOS.Methods Sixty female SD rats were randomized into six groups:control,model,Zusanli(ST36),Sanyinjiao(SP6),Guanyuan(CV4)and combination(ST36+SP6+CV4,n=10 rats/group).The PCOS model was established by gavage of Letrozole solution(1.0 mg/kg)once daily for 21 consecutive days.Rats of the control group were given 1%Carboxymethyl Cellulose(CMC,1 mg/kg).EA(2 Hz)was applied to ST36,SP6,or/and CV4 for 20 min,once daily for 14 consecutive days.The number of follicles was counted,and the ovarian structure and follicular development were observed under light microscope after H.E.stain,and the contents of serum luteinizing hormone(LH),follicle-stimulating hormone(FSH),AMH,and INHB were assayed by enzyme-linked immunosorbent assay,and the ratio of LH/FSH was calculated.The immunoactivity of LH receptor(LHR)and FSH receptor(FSHR)proteins was detected by immunohistochemistry.Results After modeling,the number of follicles at the growth stage,contents of serum LH,AMH and INHB,and ratio of LH/FSH were significantly increased,and serum FSH level and FSHR,LHR immunoactivity were remarkably decreased in the model group relevant to the control group(P<0.05,P<0.01).Following EA intervention,the number of follicles at the growth stage in the SP6,CV4 and combination groups,LH/FSH ratio and serum AMH levels in the 4 EA groups,INHB contents in the ST36,CV4 and combination groups were significantly reduced(P<0.05,P<0.01),while serum FSH contents in the 4 EA groups,FSHR immunoactivity at the early stage in the ST36 group and LHR immunoactivity at both early and late stages in the ST36 and CV4 groups were considerably increased in comparison with those of the model group(P<0.01,P<0.05).The therapeutic effect of SP6 was significantly superior to t

关 键 词：多囊卵巢综合征 电针 卵泡发育 促性腺激素 受体 

分 类 号：R245.97[医药卫生—针灸推拿学]