黄芪桂枝五物汤组方中单味药白芍的质量表征研究  

Quality characterization of Radix Paeoniae Alba in Huangqi Guizhi Wuwu decoction

作　　者：张天一 孙磊 乔善义 ZHANG Tian-yi;SUN Lei;QIAO Shan-yi(Nanjing University of Chinese Medicine,Nanjing 210023,China;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]南京中医药大学,南京210023 [2]军事科学院军事医学研究院毒物药物研究所,北京100850

出　　处：《国际药学研究杂志》2019年第6期472-478,共7页Journal of International Pharmaceutical Research

基　　金：国家“重大新药创制”科技重大专项资助项目(2013ZX09508-105)

摘　　要：目的研究黄芪桂枝五物汤中单味药白芍传统水煎剂化学成分的HPLC指纹图谱,测定其指标成分芍药苷的含量,为白芍的质量表征和控制提供科学方法,同时关联该经典名方标准颗粒与组方单味药白芍的质量。方法采用Thermo Syncronis C1(8250mm×4.6mm,5μm)色谱柱,以乙腈(A)和0.05%磷酸水溶液(B)为流动相,检测波长为230nm,柱温为30℃,流速为1.0ml/min;进样量为20μl;梯度洗脱程序为0~15min,5%A^15%A;15~45min,15%A^25%A;45~65min,25%A^50%A。结果建立了白芍传统水煎剂的HPLC指纹图谱,利用HPLC-QTOF-MS/MS技术指认了11个共有峰,并对10个不同批次白芍水煎剂指纹图谱进行了相似度评价,完成了10个批次白芍水煎剂冻干粉中芍药苷的含量测定。结论为白芍的质量表征和控制提供了科学可靠的方法,同时也为基准汤剂及颗粒剂的质量表征和控制研究提供了借鉴。Objective To establish the HPLC fingerprint of the chemical components of the decoction of Radix Paeoniae Alba(RPA)and determine the content of paeoniflorin,so as to provide a scientific method for the quality characterization and control of RPA and establish a correlation between the quality of standard granules in classical prescription and the quality of RPA.Methods Thermo Syncronis C18(250 mm×4.6 mm,5μm)chromatographic column was used,the mobile phase was constituted by acetonitrile(A)and 0.05%phosphate aqueous solution(B),the detection wavelength was 230 nm,the column temperature was 30℃,the flow rate was 1.0 ml/min,and the injection volume was 20μl;the gradient eluting procedure was 0-15 min,5%A-15%A;15-45 min,15%A-25%A;45-65 min,25%A-50%A.Results The HPLC fingerprint of RPA was established,11 common peaks were identified by HPLC-Q-TOFMS/MS,and the similarity of 10 batches were evaluated.The content determination of paeoniflorin in 10 batches of RPA decoction-lyophilized powders was also completed.Conclusion This study provides a scientific and reliable method for the quality characterization and control of RPA as well as a reference for the quality characterization and control of the standard decoction and granules.

关 键 词：白芍 芍药 高效液相 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]