转Bar基因紫花苜蓿杂交组合的筛选与评价  

Screening and Evaluation of Hybrid Combinations of the Bar Transgenic Alfalfa

作　　者：周仂 王英哲[2] 徐博[1] 谭晶 徐安凯[1,2] ZHOU Le;WANG Ying-zhe;XU Bo;Tan Jing;XU An-kai(College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin Province 130118,China;Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun,Jilin Province 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林省农业科学院,吉林长春130118

出　　处：《草地学报》2019年第5期1163-1171,共9页Acta Agrestia Sinica

基　　金：吉林省科技厅优秀青年基金项目（20190103103JH）;吉林省农业科学院结余经费（统筹部分）资助项目（y81990501）共同资助

摘　　要：为探究抗除草剂转基因紫花苜蓿(Medicago sativa)的高效杂交种选育技术。本试验以‘公农1号’作为父母本配置了1个对照组合,以转Bar基因的抗草铵膦除草剂转基因紫花苜蓿作为父本,以雄性不育系MS-GN-1A作为母本,配置了11个杂交组合。对后代植株的抗性进行Bar试纸条、PCR检测,对产量和品质性状进行评价和筛选,结果表明,试验中有7个杂交组合的后代表现优异。此试验以紫花苜蓿雄性不育系作为母本配置杂交种,使抗除草剂转基因紫花苜蓿的目标性状高效且稳定遗传,同时在此试验基础上筛选出了优良杂交后代,为良种繁育提供了一定的理论基础。To explore and validate the efficient hybrid breeding technology of herbicide-tolerant transgenic alfalfa,one control group was conducted using the alfalfa‘GN-1’as the male and female parents;11 hybrid combinations were conducted using 11 glyphosate-resistant herbicide transgenic alfalfa as the male parent and the male sterile line MS-GN-1A as the female parent.The resistance of progeny plants was tested by test paper and PCR,and the yield and quality traits were evaluated and screened.We found that the progenies of 7 hybrid combinations showed excellent performance.Our research used male sterile lines as hybrid seeds to make the target traits of herbicide-tolerant transgenic alfalfa inherit efficiently and stability.Additionally,we have screened out excellent hybrid offspring,which provides a certain theoretical basis for seed breeding.

关 键 词：转基因紫花苜蓿 雄性不育 抗草铵膦 

分 类 号：S541.9[农业科学—作物学]