染料木黄酮调控小鼠血管平滑肌细胞钙化的研究  

Study of Genistein on the regulation of calcification in vascular smooth muscle cells of mice

作　　者：沈诚[1] 赵树林[1] 袁烨[1] Shen Cheng;Zhao Shulin;Yuan Ye(Department of Thoracic Surgery,Institute of Surgery Research,Daping Hospital,Army Medical University,Chongqing 400042,China)

机构地区：[1]中国人民解放军陆军军医大学大坪医院野战外科研究所 胸外科

出　　处：《中国体外循环杂志》2019年第5期299-302,共4页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金资助项目(81370985)

摘　　要：目的观察染料木黄酮(Genistein)对小鼠血管平滑肌细胞(VSMCs)钙化的影响,并探讨其可能通过的信号通路。方法体外培养小鼠血管平滑肌细胞并构建钙化模型,分为Genistein 30μmol/L处理组、钙化组、钙化+Genistein 10μmol/L处理组、钙化+Genistein 30μmol/L处理组、钙化+Genistein 50μmol/L处理组。茜素红染色观测各组钙化情况,用荧光定量聚合酶链反应(PCR)和Western Blot检测护骨素(OPG)、α-平滑肌肌动蛋白(a-SMA)、碱性磷酸酶(ALP)、骨桥蛋白(OPN)表达。结果茜素红染色表明,Genistein能明显抑制VSMCs钙化,并呈浓度依赖性。荧光定量PCR和Western Blot结果表明,Genistein可诱导OPG、a-SMA的表达、抑制骨形成相关基因ALP、OPN的表达(P<0.05)。结论Genistein通过OPG信号通路调控小鼠VSMCs,为抑制血管钙化提供新的途径。Objective To observe the effect of Genistein on the calcification of vascular smooth muscle cells(VSMCs)of mice and to explore possible signaling pathways.Methods Vascular smooth muscle cells of mice were cultured in vitro and the calcification models were constructed.They were divided into 30μmol/L Genistein treated group,calcification group,10μmol/L Genistein treated calcification group,30μmol/L Genistein treated calcification group and 50μmol/L Genistein treated calcification group.Alizarin red staining was used to observe the calcification in each group.The expression levels of OPG,a-SMA,ALP and OPN were detected by fluorescence quantitative PCR and Western blotting.Results Alizarin red staining showed that Genistein could significantly inhibit VSMCs calcification in a concentration-dependent manner.The results of fluorescence quantitative PCR and Western blotting showed that Genistein could induce the expression of OPG and a-SMA,and inhibit the expression of ALP and OPN related to bone formation(P<0.05).Conclusion Genistein could inhibit the calcification of vascular smooth muscle cells(VSMCs)of mice through OPG/RANKL signaling pathway,which might provide a new approach for inhibition of vascular calcification.

关 键 词：染料木黄酮 血管平滑肌细胞 护骨素 钙化 小鼠 信号通路 

分 类 号：R54[医药卫生—心血管疾病] R-332[医药卫生—内科学]