乙醇对斑马鱼胚胎发育的急性毒害效应  

Acute Toxic Effect of Ethanol on Embryo Development of Zebrafish

作　　者：李爱君 张玉茹 夏雪梅 王彬容 王维香 LI Aijun;ZHANG Yuru;XIA Xuemei;WANG Binrong;WANG Weixiang(School of Food and Bioengineering,Xihua University,Chengdu 610039 China)

机构地区：[1]西华大学食品与生物工程学院

出　　处：《西华大学学报（自然科学版）》2019年第6期27-31,共5页Journal of Xihua University:Natural Science Edition

基　　金：西华大学开放课题(szjj2015-012,szjj 2017-032)

摘　　要：以斑马鱼为模型,研究乙醇对斑马鱼胚胎发育的毒害效应。用质量分数为0.5%、1%、1.3%、1.5%和2%的乙醇处理2hpf(受精后小时,hours of post-fertilization)的斑马鱼胚胎,体视显微镜观察96hpf内斑马鱼胚胎发育状况,对各暴露阶段的死亡、孵化、畸形等指标进行测量并分析,吖啶橙荧光染色法测定细胞凋亡。结果表明:随乙醇质量分数增加,斑马鱼胚胎孵化受到抑制,死亡率和畸形率提高;72 h-EC 50 、96 h-LC 50 和96 h-IC 50 分别为1.01%、1.22%和1.56%;表型毒害主要表现为胚胎心跳缓慢无力、心包水肿、眼睛变小、尾部打结、脊柱弯曲、体长变短,与胎儿酒精综合征相似。细胞凋亡研究表明,1.3%及以上体积分数的乙醇显著提高斑马鱼胚胎细胞死亡率。Using zebrafish as a model,the acute toxicity effect of ethanol on zebrafish embryo development was evaluated. The exposure concentration of ethanol was 0.5%,1%,1.3%,1.5%,2%.The embryonic development of zebrafish by 96 hpf was observed under stereo-microscope. The mortality,hatchability and deformity were measured and analyzed. The cell apoptosis was determined through acridine orange staining method.The results show that with the increase of ethanol concentration,zebrafish embryo hatching is inhibited,mortality rate and deformity rate are increased. The 72 h-EC 50 ,96 h-LC 50 and 96 h-IC 50 were 1.01%,1.22% and 1.56% respectively.The main malformations were heartbeat decrease,pericardial edema,smaller eyes,tail knots,curvature of spine and shortening of body length,similar to fetal alcohol syndrome.Apoptotic studies results show that 1.3% ethanol and above significantly caused the death of zebrafish embryonic cells.

关 键 词：乙醇 斑马鱼 胎儿酒精综合征 胚胎发育 畸变 

分 类 号：Q954.4[生物学—动物学]