试管苗玻璃化现象的研究进展  

Advances in Hyperhydricity of Plant Tissue Culture

作　　者：高弘扬 许丹芸 周良云 罗碧 杨全[1] GAO Hong-yang;XU Dan-yun;ZHOU Liang-yun;LUO Bi;YANG Quan(Guangdong Pharmaceutical University,Guangzhou Guangdong 510006)

机构地区：[1]广东药科大学

出　　处：《现代农业科技》2019年第20期52-56,59,共6页Modern Agricultural Science and Technology

摘　　要：玻璃化是植物组织培养过程中特有的一种生理失调或生理病变,普遍发生于多种植物中。玻璃化试管苗呈透明或半透明的水浸状,叶片卷曲畸形,分化及存活能力低,无法进行继代培养及驯化移栽,给种苗商业化生产造成了严重的经济损失,也限制了组织培养技术在种质资源保存及基因工程育种中的应用。目前,对玻璃化发生的机制尚没有统一的认识。本文对玻璃化现象、发生机制以及预防措施等相关研究进行了综述,以期为研究玻璃化发生发展的机制并开发有效的防控措施提供借鉴。Hyperhydricity(HH)is a physiological disorder and morphological malformation owing to stresses related to in vitro culture conditions,which often occurs in plant tissue culture.Hyperhydric plantlets show a typical glassy or vitrescent appearance,which is characterized by thick,translucent,curled,and brittle leaves.These plantlets survive very poorly when they are subcultured in fresh medium or transferred to an ex vitro environment.It can cause considerable losses in commercial micropropagation industry by reducing the quality and multiplication rate of the cultured plants.It also limits the application of tissue culture methods to the conservation of plant resources and genetic transformation of plants.Up to now,the molecular mechanism of HH is not yet clear.In this paper,research progress on the phenomena,mechanism and preventive measures of hyperhydricity were reviewed,in order to provide references for the study of the mechanism of hyperhydricity and the development of effective preventive and control measures.

关 键 词：植株组织培养 试管苗 玻璃化 发生机制 防控措施 

分 类 号：S604[农业科学—园艺学]