片仔癀调控膜联蛋白A1/VEGF通路对人肝癌细胞系HepG2作用的影响  

Pien Tze Huang promotes HepG2 apoptosis via regulating ANXA1/VEGF signaling pathway

作　　者：赖文芳[1] 黄鑫 刘俊杰 唐宇恒 林昱 林雅[1] 林国清[2] LAI Wen-fang;HUANG Xin;LIU Jun-jie;TANG Yu-heng;LIN Yu;LIN Ya;LIN Guo-qing(College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Dept ofTraditional Medicine, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学附属人民医院传统内科,福建福州350004

出　　处：《中国药理学通报》2019年第11期1534-1538,共5页Chinese Pharmacological Bulletin

基　　金：福建省自然科学基金面上项目(No 2016J01775);福建省教育厅科技类一般项目(No JAT160243)

摘　　要：目的 研究片仔癀对人肝癌细胞系 HepG2 作用的影响,并探讨其作用机制。方法 培养人肝癌细胞HepG2,将细胞分为空白对照组(Normal)、片仔癀不同浓度剂量组(浓度分别为1、10、100 mg·L -1 ),药物作用24 h后,收集细胞进行以下相关检测:MTT比色法检测不同浓度片仔癀对HepG2细胞活性的影响;集落形成实验观察细胞存活能力;Western blot法检测凋亡相关指标Bax、Bcl-2、cleaved caspase-3、cleaved caspase-9的表达,膜联蛋白A1/VEGF通路相关指标(ANXA1、VEGF、VEGFR、NF-κB p50);结果 与空白对照组比较,不同浓度片仔癀能够抑制体外人肝癌细胞株HepG2细胞活性,抑制细胞集落形成,促进HepG2细胞凋亡蛋白表达,促进细胞ANXA1蛋白表达,抑制VEGF、VEGFR表达,及抑制核蛋白NF-κBp50表达;结论 片仔癀能够抑制体外人肝癌细胞株HepG2活性,其主要作用机制与促进HepG2细胞凋亡蛋白,促进细胞ANXA1蛋白,抑制VEGF/VEGF受体,及NF-κB信号通路相关。Aim To explore the effect of Pien Tze Huang (PZH) on inhibiting HepG2 cells via regulating ANXA1/VEGF signaling pathway and the underlying mechanism. Methods HepG2 cells were treated with different concentrations (1, 10, 100 mg·L -1 ) of PZH. MTT assay and colony formation assay were used to calculate cell viability and cell survival. Western blot was used to determine the expression of Bax, Bcl-2, cleaved caspase-3, cleaved caspase-9, and ANXA1/VEGF signaling pathway protein, such as ANXA1, VEGF, VEGFR, NF-κB p50. Results Compared with normal group, different concentrations of PZH inhibited HepG2 cells in a dose-dependent manner, inhibited the colony formation, promoted the expression of apoptotic expression, promoted the expression of ANXA1 protein, inhibited the expression of VEGF, VEGFR, and NF-κB p50 as well. Conclusions PZH can inhibit the activity of HepG2 cells in vitro. Its main mechanism is related to the promotion of apoptotic protein in HepG2 cells, the promotion of cell ANXA1 protein, and the inhibition of VEGF/VEGF receptor and NF-κB signaling pathway.

关 键 词：片仔癀 HEPG2 细胞凋亡 膜联蛋白A1 VEGF受体 NF-ΚB 

分 类 号：R282.71[医药卫生—中药学] R341[医药卫生—中医学]